Document Detail

Changes in disaccharide composition of heparan sulphate fractions with increasing degrees of sulphation.
MedLine Citation:
PMID:  6221718     Owner:  NLM     Status:  MEDLINE    
Heparan sulphate by-products from the commercial manufacture of pig mucosal heparin were freed of chondroitin sulphate and fractionated according to anionic density. The fractions were treated with HNO2 at pH 1.5, and the resulting mixtures of oligosaccharides were reduced with NaB3H4 and analysed for their disaccharide composition by paper chromatography and by high-pressure liquid chromatography. The results show that the molar ratio of 2-O-sulpho-alpha-L-iduronosylanhydromannose to 6-O-sulpho-(2-O-sulpho-alpha-L-iduronosyl)anhydromannose decreased from 2.5 to 0.04 as the degree of sulphation of the fractions increased. In contrast, the molar ratio of 6-O-sulpho-(beta-D-glucuronosyl)anhydromannose to 6-O-sulpho-(alpha-L-iduronosyl)anhydromannose was approx. 2.4 in all heparan sulphate fractions and decreased to only half of this value in the most highly sulphated heparin fractions. These results are consistent with biosynthetic studies, which have shown that the N-sulpho-(2-O-sulpho-alpha-L-iduronosyl)D-glucosamine disaccharide is the metabolic precursor of the NO-disulpho-(2-O-sulpho-alpha-L-iduronosyl)-D-glucosamine disaccharide in heparin biosynthesis. The high-pressure liquid chromatography of the heparan sulphate oligosaccharides also revealed a number of unidentified oligosaccharides in the deamination mixtures.
S R Delaney; H E Conrad
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Biochemical journal     Volume:  209     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-06-10     Completed Date:  1983-06-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  315-22     Citation Subset:  IM    
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MeSH Terms
Blood Coagulation / drug effects
Chemical Phenomena
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Disaccharides / analysis*
Glycosaminoglycans* / pharmacology
Heparin / pharmacology
Heparitin Sulfate* / pharmacology
Nitrous Acid
Sulfates / analysis*
Grant Support
Reg. No./Substance:
0/Disaccharides; 0/Glycosaminoglycans; 0/Sulfates; 7782-77-6/Nitrous Acid; 9005-49-6/Heparin; 9050-30-0/Heparitin Sulfate

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