| Changes in cyclin dependent kinase expression and activity accompanying lens fiber cell differentiation. | |
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MedLine Citation:
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PMID: 10620399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies from this laboratory have shown that differentiating lens fiber cells contain two active cyclin dependent kinases (Cdks), Cdk1 and Cdk5. The present study was undertaken to explore the expression and regulation of six additional members of the Cdk family (Cdk2, Cdk3, Cdk4, Cdk6, Cdk7 and Cdk8) during lens differentiation. Differentiating lens fiber cells were separated from lens epithelial cells by microdissection of developing rat lenses [embryonic day 16 (E16) to postnatal day 8 (P8)] and Cdk expression was assessed by RT-PCR and immunoblotting. Two Cdks (Cdk3 and Cdk6) were not expressed in lens fiber cells or epithelial cells during this developmental period. In the lens epithelium, we detected proteins and mRNAs corresponding to all other Cdks examined (Cdk2, Cdk4, Cdk7, Cdk8) throughout this developmental period. Epithelial cells showed significant Cdk2 activity, which decreased with developmental age, but no significant activity was detected for Cdk4, Cdk7, or Cdk8. Fiber cells contained all four Cdk proteins and the corresponding Cdk mRNAs except for Cdk2 mRNA. None of the Cdks examined showed significant kinase activity in fiber cells. Immunoprecipitates of Cdk2 and Cdk4 from fiber cells contained p57(kip2), supporting the view that this Cdk inhibitor blocks the activity of these Cdks in lens fibers. In contrast, p57(kip2)did not co-immunoprecipitate with Cdk5 from lens fibers. These findings suggest that the differential affinity of p57(kip2)for members of the Cdk family may provide a mechanism for specific regulation of individual Cdks during fiber cell differentiation. |
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Authors:
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C Y Gao; A M Rampalli; H C Cai; H Y He; P S Zelenka |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental eye research Volume: 69 ISSN: 0014-4835 ISO Abbreviation: Exp. Eye Res. Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2000-02-24 Completed Date: 2000-02-24 Revised Date: 2012-06-25 |
Medline Journal Info:
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Nlm Unique ID: 0370707 Medline TA: Exp Eye Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 695-703 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Academic Press. |
Affiliation:
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Laboratory of Molecular and Developmental Biology, NEI/NIH, Bethesda, MD, 20892-2730, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals CDC2-CDC28 Kinases* Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinase 4 Cyclin-Dependent Kinases / genetics*, metabolism DNA Primers Electrophoresis, Polyacrylamide Gel Embryonic Induction Epithelial Cells / metabolism Gene Expression Immunoblotting Lens, Crystalline / cytology, embryology*, metabolism Precipitin Tests Protein-Serine-Threonine Kinases / genetics*, metabolism Proto-Oncogene Proteins* Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Proto-Oncogene Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/Cdk2 protein, rat; EC 2.7.11.22/Cdk4 protein, rat; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinase 4; EC 2.7.11.22/Cyclin-Dependent Kinases; EC 2.7.11.22/cyclin-dependent kinase-activating kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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