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Changes in the components of extracellular matrix and in growth properties of cultured aortic smooth muscle cells upon ascorbate feeding.
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MedLine Citation:
PMID:  7061590     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Culture conditions can modify the composition of the extracellular matrix of cultured calf aortas smooth muscle cells. In the absence of ascorbate the major components of the matrix are microfibrillar proteins; deposition of collagen occurs upon ascorbate supplementation and, with increased time of exposure of cells to ascorbate, collagen becomes the dominant protein of the extracellular matrix (greater than 80%). Collagen accumulation follows a sigmoidal time-course, suggesting that it is a cooperative phenomenon. Covalent crosslinks are not required for collagen accumulation in the matrix. Microfibrillar proteins and increased amounts of proteoglycans and fibronectin accumulate concurrently with collagen but elastin deposition was not observed either with or without ascorbate feeding. Addition of ascorbate leads to a general stimulation of incorporation of [14C]proline into cellular protein and to changes in cell growth parameters and morphology: cell-doubling time decreases from 62 to 47 h and plating efficiency increases approximately fourfold. We conclude that the composition of the extracellular matrix assembled by cultured cells is subject to experimental manipulation and that changes in endogenously deposited matrix may have significant effects on cellular functions.
Authors:
E Schwartz; R S Bienkowski; B Coltoff-Schiller; S Goldfischer; O O Blumenfeld
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  92     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1982 Feb 
Date Detail:
Created Date:  1982-05-27     Completed Date:  1982-05-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  462-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascorbic Acid / pharmacology*
Cattle
Cell Adhesion
Cell Division
Cells, Cultured
Collagen / metabolism
Extracellular Space / physiology*
Fibronectins / metabolism
Muscle, Smooth, Vascular / cytology,  physiology*
Proteoglycans / metabolism
Grant Support
ID/Acronym/Agency:
AG00374/AG/NIA NIH HHS; HL 21756/HL/NHLBI NIH HHS; HL 22729/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Fibronectins; 0/Proteoglycans; 50-81-7/Ascorbic Acid; 9007-34-5/Collagen
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 2 Year: 1982
Volume: 92 Issue: 2
First Page: 462 Last Page: 470
ID: 2112078
Publisher Id: 82142724
PubMed Id: 7061590

Changes in the components of extracellular matrix and in growth properties of cultured aortic smooth muscle cells upon ascorbate feeding


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