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Changes in the colchicine susceptibility of microtubules associated with neurite outgrowth: studies with nerve growth factor-responsive PC12 pheochromocytoma cells.
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MedLine Citation:
PMID:  7142295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The PC12 line of nerve growth factor (NGF)-responsive rat pheochromocytoma cells was used as a model system to determine whether properties of microtubules change during neurite growth and maturation. In the absence of NGF, PC12 cells lack processes. After several days with NGF, PC12 cells begin extending neurites and, by 2-3 wk with NGF, PC12 cells have long (approximately 1 mm), highly branched neurites. We examined the effect of colchicine on microtubules of PC12 cells grown without NGF or with NGF for 1 or 21 d. PC12 cells grown under the various conditions were exposed to 50 microM colchicine for 1 or 6 h, and were then assayed for their content of polymerized tubulin using a biochemical assay. Microtubule levels in drug-treated cultures were compared to those in non-drug-treated control sister cultures. PC12 cells grown without NGF or with NGF for 1 d were depleted of MT by 1 h with colchicine. In contrast, microtubule levels in long-term NGF-treated cells exposed to colchicine for 6 h were reduced to only approximately 57% of those in control cells. Control experiments indicated that the observed differential susceptibility to colchicine was not due to differences in colchicine uptake or to the effects of colchicine on cell viability. These observations suggest that microtubules of PC12 cells grown without NGF or with NGF for 21 d differ in their properties. Such differences may be related to one or more of the changes in structure and/or motility that result from treatment with NGF.
Authors:
M M Black; L A Greene
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  95     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1982 Nov 
Date Detail:
Created Date:  1983-01-19     Completed Date:  1983-01-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  379-86     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Axons / physiology*,  ultrastructure
Blood
Cell Line
Cell Survival / drug effects
Colchicine / metabolism,  pharmacology*
Culture Media
Microtubules / drug effects*
Nerve Growth Factors / pharmacology*
Pheochromocytoma
Polymers
Rats
Tubulin
Grant Support
ID/Acronym/Agency:
NS 16036/NS/NINDS NIH HHS; NS 17681/NS/NINDS NIH HHS; S 07 RR054170/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Nerve Growth Factors; 0/Polymers; 0/Tubulin; 64-86-8/Colchicine
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 11 Year: 1982
Volume: 95 Issue: 2
First Page: 379 Last Page: 386
ID: 2112955
Publisher Id: 83057142
PubMed Id: 7142295

Changes in the colchicine susceptibility of microtubules associated with neurite outgrowth: studies with nerve growth factor-responsive PC12 pheochromocytoma cells


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