Document Detail

Changes in circulating carcinoma cells in patients with metastatic prostate cancer correlate with disease status.
MedLine Citation:
PMID:  11549486     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To investigate the diurnal variations in circulating tumor cells (CTCs) in metastatic carcinoma of the prostate (CAP) and to determine whether the change in CTCs correlated with disease progression. METHODS: Samples were prepared by immunomagnetic selection of cells from 7 mL of blood targeting the epithelial cell adhesion molecule and differential fluorescent labeling of the collected cells using a nucleic acid dye, antibodies directed against the common leukocyte (CD45), and cytokeratin antigens. Events that stained with the nucleic acid dye and expressed cytokeratin but lacked CD45 were defined as CTCs by multiparameter flow cytometry. RESULTS: Male controls (n = 22) exhibited 0.8 +/- 1.2 events per 7 mL blood compared with 5.9 +/- 4.7 in 10 samples from patients with localized CAP and 46.6 +/- 65.6 events in 10 samples from patients with metastatic CAP. Diurnal testing of 8 cases demonstrated stable levels of CTCs. Ten patients were serially analyzed during a 6-month period for serum prostate-specific antigen and CTCs. The correlation between the prostate-specific antigen level and CTC number was fair. Slow disease progression was found in 4 patients with low CTC numbers (3.0 +/- 3) but it was significantly higher than the control group (P <0.002). Rapid disease progression occurred in 6 patients who demonstrated high CTC numbers (68.5 +/- 71.9). Two patients received chemotherapy that caused substantial fluctuations in the CTCs with less pronounced changes in the prostate-specific antigen level. CONCLUSIONS: We conclude that the level of CTCs can be quantified in the circulation of patients with metastatic CAP and that the change in CTCs correlates with disease progression with no diurnal variations.
J G Moreno; S M O'Hara; S Gross; G Doyle; H Fritsche; L G Gomella; L W Terstappen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Urology     Volume:  58     ISSN:  1527-9995     ISO Abbreviation:  Urology     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-10     Completed Date:  2001-12-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0366151     Medline TA:  Urology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  386-92     Citation Subset:  IM    
Thomas Jefferson University Hospital, Philadelphia, Pennsylvania, USA.
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MeSH Terms
Aged, 80 and over
Circadian Rhythm / physiology*
Disease Progression
Flow Cytometry
Immunomagnetic Separation / methods
Middle Aged
Neoplastic Cells, Circulating / pathology*
Prostatic Neoplasms / blood,  pathology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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