Document Detail

Changes in cellular autophagic capacity during azaserine-initiated pancreatic carcinogenesis.
MedLine Citation:
PMID:  11693989     Owner:  NLM     Status:  MEDLINE    
Growth regulation is a crucial event in tumour progression. Surprisingly, relatively few papers have dealt with the catabolic side of regulation, and there are practically no data regarding the autophagic process during tumour development. We approach this problem by morphometrical investigation into the possible changes of autophagic activity during the progression of rat pancreatic adenocarcinoma induced by azaserine. In the present study, autophagic capacity of the azaserine-induced premalignant and malignant cells were characterised and compared to the respective host tissue cells of the rat pancreas and to the acinar cells in other stages of tumour development. Using vinblastine (VBL) as an enhancer, and cycloheximide (CHI) as an inhibitor of autophagic segregation we observed that autophagic capacity of premalignant cells (month 6 and 10 after initiation) is much higher than in the host tissue cells. We found a sharp decrease in self-digesting capacity in adenocarcinoma cells (month 20) where VBL induced a minimal accumulation of autophagic vacuoles which was, surprisingly, not inhibited by CHI, i.e. the CHI-sensitive regulatory step was lost. The changes in autophagic capacity are probably associated to specific steps of tumour progression in our system.
S Tóth; K Nagy; Z Palfia; G Réz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biologica Hungarica     Volume:  52     ISSN:  0236-5383     ISO Abbreviation:  Acta. Biol. Hung.     Publication Date:  2001  
Date Detail:
Created Date:  2001-11-05     Completed Date:  2002-04-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8404358     Medline TA:  Acta Biol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  393-401     Citation Subset:  IM    
Department of General Zoology, Eötvös Lorand University, Budapest, Hungary.
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MeSH Terms
Adenocarcinoma / chemically induced,  etiology,  physiopathology
Adenoma / chemically induced,  physiopathology
Autophagy* / drug effects
Azaserine / toxicity*
Carcinoma in Situ / chemically induced,  physiopathology
Cycloheximide / pharmacology
Pancreatic Neoplasms / chemically induced*,  etiology,  physiopathology*
Rats, Wistar
Time Factors
Vinblastine / pharmacology
Reg. No./Substance:
115-02-6/Azaserine; 66-81-9/Cycloheximide; 865-21-4/Vinblastine

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