Document Detail


Changes in cell wall ultrastructure induced by sudden flooding at 25{degrees}C in Pisum sativum (Fabaceae) primary roots.
MedLine Citation:
PMID:  21632404     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Cellular degeneration is essential for many developmental and stress acclimation processes. Undifferentiated parenchymatous cells in the central vascular cylinder of pea primary roots degenerate under hypoxic conditions created by flooding at temperatures >15°C, forming a long vascular cavity that seems to provide a conduit for longitudinal oxygen transport in the roots. We show that specific changes in the cell wall ultrastructure accompanied previously detected cytoplasmic and organellar degradation in the cavity-forming roots. The degenerating cells had thinner primary cell walls, less electron-dense middle lamellae, and less abundant cell wall homogalacturonans in altered patterns, compared to healthy cells of roots grown under cold, nonflooded conditions. Cellular breakdown and changes in wall ultrastructure, however, remained confined to cells within a 50-μm radius around the root center, even after full development of the cavity. Cells farther away maintained cellular integrity and had signs of wall synthesis, perhaps from tight regulation of wall metabolism over short distances. These observations suggest that the cell degeneration might involve programmed cell death. We also show that warm, nonflooded or cold, flooded conditions that typically do not induce vascular cavity formation can also induce variations in cell wall ultrastructure.
Authors:
Purbasha Sarkar; Teruo Niki; Daniel K Gladish
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of botany     Volume:  95     ISSN:  0002-9122     ISO Abbreviation:  Am. J. Bot.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2011-06-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370467     Medline TA:  Am J Bot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  782-92     Citation Subset:  -    
Affiliation:
Department of Botany, Miami University, Oxford, Ohio 45056 USA.
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