| Changes in cell wall composition of deformed ras1- cells of Schizosaccharomyces pombe. | |
| | |
MedLine Citation:
|
PMID: 8763147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Disruption of the Schizosaccharomyces pombe ras1 gene results in a morphological transformation to large spheres, in contrast to wild-type cells which grow as rods. Chemical analysis of isolated cell walls showed no significant changes in saccharide content but an increase in protein and phosphate contents in ras1- walls relative to parent walls. Polymers tightly bound to the cell wall were solubilized by SDS treatment. Several compounds with molar mass ranging from 22 to 130 kDa and more were resolved by gel filtration and SDS-PAGE. Among low-molar-mass species, a component moving as a band at 31 kDa was conspicuous in ras1- cell walls. It was solubilized by heating in Tris-HCl buffer and shown to have a beta-1,3-glucanase activity against laminarin. The level of the enzyme was by 30% higher in the ras1- cell wall than in the wild-type cell wall. This enzyme may participate in the remodelling of the rigid glucan network and account (at least partially) for the aberrant cell shape. The ras1- cell wall contained a high level of charged polymers, especially phosphoproteins, raising the appealing possibility that ras1- is involved in a putative kinase cascade required to sense and respond to external stimuli destined for the cell wall. Although the present study shows that ras1 loss of function and altered cell wall composition are closely linked defects, it has still to be shown that the ras1 protein is directly involved in alterations found in the mutant cell walls. |
| | |
Authors:
|
N Harmouch; A Pichová; J Coulon; E Streiblová; R Bonaly |
Related Documents
:
|
4120067 - Electron microscopy of some special cell contracts in yeasts. 20190047 - Characterization of acp, a peptidoglycan hydrolase of clostridium perfringens with n-ac... 6625307 - Ultrastructure of the equine hoof wall secondary epidermal lamellae. 6490737 - The three-dimensional structure of the cell wall glycoprotein of chlorogonium elongatum. 16903207 - Regulation of s phase. 11262197 - Transcriptional activation of prion protein gene in growth-arrested and differentiated ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Folia microbiologica Volume: 40 ISSN: 0015-5632 ISO Abbreviation: Folia Microbiol. (Praha) Publication Date: 1995 |
Date Detail:
|
Created Date: 1996-10-18 Completed Date: 1996-10-18 Revised Date: 2005-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0376757 Medline TA: Folia Microbiol (Praha) Country: CZECH REPUBLIC |
Other Details:
|
Languages: eng Pagination: 519-27 Citation Subset: IM |
Affiliation:
|
Laboratoire de Biochimie Microbienne, Faculté des Sciences Pharmaceutiques et Biologiques, Nancy, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Carbohydrates
/
analysis Cell Wall / chemistry* Fungal Proteins / analysis, genetics* Glucan 1,3-beta-Glucosidase Glycoproteins / analysis Schizosaccharomyces / chemistry*, genetics Schizosaccharomyces pombe Proteins* beta-Glucosidase / analysis ras Proteins / genetics* |
| Chemical | |
Reg. No./Substance:
|
0/Carbohydrates; 0/Fungal Proteins; 0/Glycoproteins; 0/Schizosaccharomyces pombe Proteins; EC 3.2.1.21/beta-Glucosidase; EC 3.2.1.58/Glucan 1,3-beta-Glucosidase; EC 3.6.5.2/Ras1 protein, S pombe; EC 3.6.5.2/ras Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Influence of ethanol on the lipid content and fatty acid composition of Saccharomyces cerevisiae.
Next Document: Purification and partial immunochemical characterization of proteins of fimbriae F107 from Escherich...