Document Detail

Changes in cell surface charge and transmembrane potential accompanying neoplastic transformation of rat kidney cells.
MedLine Citation:
PMID:  3828335     Owner:  NLM     Status:  MEDLINE    
Free flow electrophoresis measurements have been used to determine the surface charge density of normal rat kidney (NRK) cells and a clone of NRK, designated as 6m2, that exhibit a transformed phenotype at 33 degrees C and a non-transformed phenotype at 39 degrees C. A clone of 6m2, designated 54-5A4, which is transformed at both 33 degrees C and 39 degrees C was also studied. A surface charge density of -1.42 microC/cm2 was obtained for the NRK and non-transformed 6m2 cells at 39 degrees C, whereas at 33 degrees C values of -1.85 and -1.78 microC/cm2 were determined for the transformed 6m2 and 54-5A4 cells, respectively. It was found that 72% of the increased charge that appeared on the transformed 6m2 cells compared with the non-transformed 6m2 cells was RNAase sensitive. The time-dependent decrease in surface charge that accompanied the shift of the 6m2 cells from their transformed to non-transformed state was found to mirror the increase in transmembrane potential previously reported using a fluorescent dye technique, and was also comparable to the reported temporal changes in their morphology and virally-coded protein content.
J A Price; R Pethig; C N Lai; F F Becker; P R Gascoyne; A Szent-Györgyi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  898     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-05-13     Completed Date:  1987-05-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  129-36     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Membrane / physiology*
Cell Transformation, Neoplastic*
Kidney / physiopathology*
Membrane Potentials
Ribonucleases / pharmacology
Reg. No./Substance:
EC 3.1.-/Ribonucleases

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