Document Detail


Changes in cell shape and anchorage in relation to the restriction point.
MedLine Citation:
PMID:  15534858     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The restriction point (R) separates the G1 phase of continuously cycling cells into two functionally different parts. The first part, G1-pm, represents the growth factor dependent post-mitotic interval from mitosis to R, which is of constant length (3-4 h). The second part, G1-ps, represents the growth factor independent, pre-S phase interval of G1 that lasts from R to S and that varies in time from 1 to 10 h. G1-pm cells rapidly exit (within 1 h) from the cell cycle and enter G0 as a response to serum withdrawal. The finding that R occurs at a set time after mitosis indicates that R may be related to the metabolic and/or structural changes that the cell underwent during the previous mitosis. We have recently shown that phosphorylation of the retinoblastoma tumor suppressor protein (pRb) is not the molecular mechanism behind R, as has been suggested previously. Here, we present an alternative explanation for R. In the present study, we applied a single cell approach using time-lapse analysis, which revealed that upon serum starvation the G1-pm cells rapidly underwent a transient change in cell shape from flat to spherical before exiting to G0. Platelet derived growth factor (PDGF) counteracted this change in shape and also prevented exit to G0 to the same extent. Furthermore epidermal growth factor (EGF) and insulin like growth factor (IGF-1), which only partially counteracted this change, only partially counteracts exit to G0. These data clearly indicate a direct link between change in cell shape and exit to G0 in G1-cells that have not passed R.
Authors:
Hanna-Stina Martinsson; Peter Zickert; Maria Starborg; Olle Larsson; Anders Zetterberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  203     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-02-01     Completed Date:  2005-04-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  27-34     Citation Subset:  IM    
Copyright Information:
2004 Wiley-Liss, Inc.
Affiliation:
Karolinska Institutet, Department of Oncology-Pathology, Karolinska Hospital/CCK R8:04, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Anticoagulants / pharmacology
Blood Proteins / pharmacology
Cell Adhesion / drug effects,  physiology*
Cell Shape / drug effects,  physiology*
Culture Media, Serum-Free / pharmacology
Cytoskeleton / physiology
Fibroblasts / cytology*,  drug effects
G1 Phase / physiology*
Humans
Mice
Platelet-Derived Growth Factor / pharmacology
Chemical
Reg. No./Substance:
0/Anticoagulants; 0/Blood Proteins; 0/Culture Media, Serum-Free; 0/Platelet-Derived Growth Factor; 0/platelet-derived growth factor BB

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