Document Detail


Changes in cell morphology affect the quantification of intercellular communication.
MedLine Citation:
PMID:  17548199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The scrape loading and dye transfer (SL/DT) method is a functional assay widely used to evaluate the level of the intercellular communication via gap junction. Initially, the evaluation of the degree of communication was done by counting the rows of fluorescent cells from the scrape. Its substitution by fluorescence distance or area, determinated by morphometric softwares, was proposed as the best way of communication measurement. Considering that normal cells change their morphology during different phases of growth in monolayer cultures we proposed to evaluate the possible influence of the cell morphology changes on the quantification of intercellular communication when the SL/DT method is used. The present data indicates that GJIC capacity should be rather quantified by counting the fluorescent cell rows than by the measurement of the fluorescence distance or area, when the goal is to evaluate the cell communication along different phases of cell culture. Normal cells growing in monolayer culture decrease their diameter according to their final density of saturation, impairing the quantification of GJIC capacity by morphometric analysis.
Authors:
Beatriz Brandão Vaz-de-Lima; Marisa Ionta; Glaucia M Machado-Santelli
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-03
Journal Detail:
Title:  Micron (Oxford, England : 1993)     Volume:  39     ISSN:  0968-4328     ISO Abbreviation:  Micron     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-16     Completed Date:  2008-09-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312850     Medline TA:  Micron     Country:  England    
Other Details:
Languages:  eng     Pagination:  631-4     Citation Subset:  IM    
Affiliation:
Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Biologia Celular e do Desenvolvimento, Sao Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Communication / physiology*
Cell Count
Cell Line
Fluorescent Dyes / metabolism
Gap Junctions / physiology*
Isoquinolines / metabolism
Liver / cytology*,  ultrastructure*
Microscopy, Fluorescence
Rats
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Isoquinolines; 77944-88-8/lucifer yellow

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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