Document Detail


Changes in beta-adrenoceptors and leucocyte subpopulations after physical exercise in normal subjects.
MedLine Citation:
PMID:  2839777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Seven healthy volunteers were subjected to standardized bicycle ergometry. Before and at the end of exercise, leucocyte and lymphocyte subset distribution was assessed by immunofluorescence labelling with monoclonal antibodies and the cytofluorograf. Competition binding studies were performed on mononuclear leucocytes with a fixed amount of the radioligand 125I-(-)-cyanopindolol (125I-CYP) and increasing concentrations of the hydrophilic ligand (-)4-(3-tertiary-butylamino-2-hydroxypropoxy)-benzimidazol-2-one hydrochloride (CGP-12177). Total numbers of beta-adrenoceptors per cell and the receptor-ligand affinities were then derived by computer analysis. In separate experiments with blood obtained from resting subjects, beta-adrenoceptor numbers of lymphocyte subsets, which had been sorted by the fluorescence activated cell sorter, were determined by saturation binding of 125I-CYP. During exercise there was a twofold increase in total leucocyte numbers. The ratio between monocytes (4%) and lymphocytes (55%) remained constant, but the composition of lymphocyte subsets had changed. A twofold increase was observed for the lymphocyte population carrying the Leu-7 and the CD8 antigens or only the Leu-7 antigen, whereas the number of B cells and CD4 positive T cells increased only slightly. The lymphocyte phenotype changes appeared after 3 min, reached a maximum at the end of ergometry and had disappeared 30 min after exercise. Exercise led to a doubling of the number of beta-adrenoceptors in unseparated mononuclear leucocytes from 240 +/- 46 to 535 +/- 190 sites per cell (SD, n = 6). beta-Adrenoceptor numbers were higher on sorted Leu-7 positive cells (KD 14 +/- 6 pmol/l, Bmax 1174 +/- 233 sites/cell) and on CD8 positive cells (KD 145 +/- 79 pmol/l, Bmax 1577 +/- 670 sites/cell) than on monocytes (KD 39 +/- 31 pmol/l, 647 +/- 91 sites/cell). Very low specific 125I-CYP binding was found on Leu-7 and CD8 negative cells (less than 0.2 pmol/l). Before exercise the displacement curves with CGP-12177 fitted best a single site model, whereas after exercise 12% of the receptors were in a low affinity state for CGP-12177 indicating internalized receptors. (ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
R Landmann; M Portenier; M Staehelin; M Wesp; R Box
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Naunyn-Schmiedeberg's archives of pharmacology     Volume:  337     ISSN:  0028-1298     ISO Abbreviation:  Naunyn Schmiedebergs Arch. Pharmacol.     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-08-22     Completed Date:  1988-08-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0326264     Medline TA:  Naunyn Schmiedebergs Arch Pharmacol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  261-6     Citation Subset:  IM    
Affiliation:
Department of Research, Kantonsspital, Basel, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Adult
Catecholamines / blood
Female
Humans
Iodine Radioisotopes / diagnostic use
Iodocyanopindolol
Leukocytes / metabolism*
Male
Phenotype
Physical Exertion*
Pindolol / analogs & derivatives,  diagnostic use
Receptors, Adrenergic, beta / metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Catecholamines; 0/Iodine Radioisotopes; 0/Receptors, Adrenergic, beta; 13523-86-9/Pindolol; 83498-72-0/Iodocyanopindolol

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