Document Detail

Changes in arachidonic acid levels and formation and in lipid synthesis in the human neuroblastoma SK-N-BE during retinoic acid-induced differentiation.
MedLine Citation:
PMID:  8764579     Owner:  NLM     Status:  MEDLINE    
We observed that retinoic acid, which differentiates the human neuroblastoma SK-N-BE into mature neurons, induced an elevation in levels of polyunsaturated fatty acids, especially arachidonic acid (20:4 n-6). This effect was not induced by phorbol myristate acetate, another differentiating agent. We then explored the effects of retinoic acid on the formation of arachidonic acid and of docosahexaenoic acid from precursors and on the de novo lipid synthesis from acetate at various stages of differentiation, which was assessed by morphological (cell number and neurite outgrowth) and biochemical (protein content and thymidine incorporation) criteria. At 3 days of incubation with retinoic acid, in the n-6 series, total conversion of linoleic acid, especially to 20:3 n-6, was elevated, in association with preferential incorporation of acetate into phospholipids; in contrast, at 8 days, synthesis of 20-carbon polyunsaturated fatty acids declined, in association with enhanced incorporation in triglycerides. In the n-3 series, eicosapentaenoic acid was converted to docosahexaenoic acid in SK-N-BE, but the conversion was not affected by retinoic acid. During the early stage of neuronal differentiation, therefore, enhanced production of 20-carbon polyunsaturated fatty acids from their precursors occurred, and newly formed fatty acids were preferentially incorporated in phospholipids, possibly in association with membrane deposition. When differentiation was completed, arachidonic acid formation and incorporation of acetate in phospholipids and cholesterol declined with enhanced labeling of storage lipids.
A Petroni; M Blasevich; N Papini; P La Spada; C Galli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  67     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-09-19     Completed Date:  1996-09-19     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  549-56     Citation Subset:  IM    
Institute of Pharmacologial Sciences, University of Milan, Italy.
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MeSH Terms
Arachidonic Acid / metabolism*
Cell Differentiation / drug effects
Cells, Cultured
Cerebral Cortex / cytology
Fatty Acids, Unsaturated / metabolism
Lipid Metabolism*
Nerve Tissue Proteins / metabolism
Neuroblastoma / metabolism*
Neurons / cytology,  metabolism*
Tretinoin / pharmacology
Tumor Cells, Cultured
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Nerve Tissue Proteins; 302-79-4/Tretinoin; 506-32-1/Arachidonic Acid

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