| Changes in amount of ADM mRNA and RAMP2 mRNA in calcified vascular smooth muscle cells. | |
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MedLine Citation:
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PMID: 12668214 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This work was aimed to explore the changes and significance of adrenomedullin (ADM) mRNA and receptor activity modifying protein 2 (RAMP2) mRNA in calcified vascular smooth muscle cells (VSMCs). Calcification of cultured rat VSMCs was produced by incubation with beta-glycerophosphate. Content of ADM released by VSMCs was measured by radioimmunoassay (RIA). The amount of ADM mRNA and RAMP2 mRNA was determined by competitive quantitative RT-PCR. The intracellular calcium content, alkaline phosphatases activity and cellular (45)Ca(2+)-uptake were determined. The results showed that the content of calcium, (45)Ca(2+)-uptake and alkaline phosphatases activity in calcified VSMCs were increased by 118%, 174% and seven-fold (all P<0.01), respectively, compared with control VSMCs. Content of ADM in medium was increased by 99% (P<0.01). Furthermore, it was found that the amount of ADM mRNA and RAMP2 mRNA in calcified cells was elevated by 78 and 56% (all P<0.05), respectively, compared with control. The elevated levels of RAMP2 mRNA were in positive correlation with ADM mRNA (r=0.76, P<0.05) in calcified VSMCs. In conclusion, calcified VSMCs generated an increased amount of ADM, and up-regulated gene expressions of ADM and RAMP2. |
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Authors:
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Yong Fen Qi; Shu Heng Wang; Bao Hong Zhang; Ding Fang Bu; Tang Chao Shu; Jun Bao Du |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Peptides Volume: 24 ISSN: 0196-9781 ISO Abbreviation: Peptides Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-04-01 Completed Date: 2004-01-12 Revised Date: 2010-12-01 |
Medline Journal Info:
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Nlm Unique ID: 8008690 Medline TA: Peptides Country: United States |
Other Details:
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Languages: eng Pagination: 287-94 Citation Subset: IM |
Affiliation:
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Institute of Cardiovascular Diseases, Peking University First Hospital, 100034, PR, Beijing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adrenomedullin Alkaline Phosphatase / metabolism Animals Calcium / metabolism Calcium Chloride / pharmacology Cells, Cultured Glycerophosphates / chemistry Intracellular Signaling Peptides and Proteins Membrane Proteins / genetics* Muscle, Smooth, Vascular / drug effects, metabolism*, pathology Peptides / genetics* RNA, Messenger / genetics, metabolism* Radioimmunoassay Rats Rats, Wistar Receptor Activity-Modifying Proteins Reverse Transcriptase Polymerase Chain Reaction Up-Regulation / drug effects, genetics |
| Chemical | |
Reg. No./Substance:
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0/Glycerophosphates; 0/Intracellular Signaling Peptides and Proteins; 0/Membrane Proteins; 0/Peptides; 0/RNA, Messenger; 0/Ramp2 protein, rat; 0/Receptor Activity-Modifying Proteins; 10043-52-4/Calcium Chloride; 148498-78-6/Adrenomedullin; 17181-54-3/beta-glycerophosphoric acid; 7440-70-2/Calcium; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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