| Changes in adrenocortical lipid fluidity of hyperfunctioning human adrenals. | |
| | |
MedLine Citation:
|
PMID: 1562555 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Lipid droplets, the storage places of cholesterol in adrenocortical cells, exhibit a relatively uniform appearance studied by the electron microscope but they are heterogeneous in respect of their optical polarizing properties. Optical birefringency was studied in cryosections of normal and hyperfunctioning adrenal cortex by a polarizing microscope, equipped with a cold/hot stage working in the temperature range from -40 to 40 degrees C. The majority of lipid droplets in normal adrenal cortex were optically anisotropic in each cortical zone at room temperature (22 degrees C) indicating a long-range molecular order of the lipid components. The lipids of the zona glomerulosa, in the cases of Conn's and Bartter's syndromes, became anisotropic when the temperature was lowered below ambient. The birefringency of the lipids of the zona fasciculata in the case of Cushing's disease was observed at temperatures below -10 degrees C indicating ordered packing of the components of lipid droplets at this temperature. Thus the lipids were more fluid in the hyperfunctioning, hormone-producing cells--this may represent an optimal precondition for their mobilization and processing by the hydrolyzing enzyme system. The changes in fluidity of the intracellular lipids can be attributed to different functional states in the adrenal cortex. Study of the thermotropic phase transitions of the lipid droplets by polarizing microscopy may be a useful additional method for the diagnosis of some adrenocortical diseases. |
| | |
Authors:
|
E Orsó; I E Tóth; D Szabó; G Szilágyi |
Related Documents
:
|
2023865 - Polymorphic behavior of sprayed lipid micropellets and its evaluation by differential s... 20816065 - Chemical versus mechanical perturbations on the protonation state of arginine in comple... 17546665 - Ph-dependent interactions of indinavir and lipids in nanoparticles and their ability to... 9606005 - Structural requirements for cationic lipid mediated phosphorothioate oligonucleotides d... 10408775 - Temperature and ph effects on single-strand conformation polymorphism analysis by capil... 12502085 - Potentiometric pvc membrane sensor for the determination of scopolamine in some pharmac... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of steroid biochemistry and molecular biology Volume: 41 ISSN: 0960-0760 ISO Abbreviation: J. Steroid Biochem. Mol. Biol. Publication Date: 1992 Mar |
Date Detail:
|
Created Date: 1992-05-18 Completed Date: 1992-05-18 Revised Date: 2005-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 9015483 Medline TA: J Steroid Biochem Mol Biol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 791-3 Citation Subset: IM |
Affiliation:
|
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenal Cortex
/
metabolism,
pathology* Adrenocortical Hyperfunction / metabolism*, pathology, surgery Adult Birefringence Cholesterol / metabolism* Female Freezing Humans Lipid Metabolism* Male Microscopy, Polarization Middle Aged Organ Specificity Reference Values Thermodynamics |
| Chemical | |
Reg. No./Substance:
|
57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Functional expression of cDNAs for bovine 11 beta-hydroxylase-aldosterone synthases, P450(11 beta)-2...
Next Document: Cortisol and testosterone: inhibitory agents of the mineralocorticoid pathway. Is there a physiopath...