Document Detail

Changes in MMPs and inflammatory cells in experimental gingivitis.
MedLine Citation:
PMID:  19085832     Owner:  NLM     Status:  MEDLINE    
In periodontal disease, extensive disorganization of the extracellular matrix promotes the loss of adhesion between the teeth and periodontium. A previous study suggested a reduction in the area occupied by collagen in the gingiva, during the first week of periodontal disease induction, however, the remaining fibers were more compact and thicker. Therefore, it was decided to investigate which of the MMP-2, -9, -14 and RECK, an MMP inhibitor, were involved in these modifications taking place in early gingivitis induced by ligature. The results of gene expression analysis indicated no changes for RECK. MMP-14 showed a reduction at 7 days of inflammation, and there was an immediate increase in MMP-2 gene expression and enzymatic activity, apparently by the stimulation of resident cells such as fibroblasts. A peak of MMP-9 expression 5 days after ligature followed after the peak of enzymatic activity found two days earlier. This pattern was consistent with the kinetics of macrophage and neutrophil recruitment. Immunohistochemistry suggested that MMP-9 was produced by both resident and inflammatory cells. Based on this evidence, it is suggested that extracellular matrix remodeling is related to MMP-2 and -9 production and activation. This allowed us to conclude that the host inflammatory response represents a significant factor for the advance of periodontal diseases.
Márcio Lorencini; Juliete A F Silva; Cristiane L R de la Hoz; Hernandes F Carvalho; Dagmar R Stach-Machado
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histology and histopathology     Volume:  24     ISSN:  1699-5848     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-02-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  157-66     Citation Subset:  IM    
Department of Microbiology and Immunology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
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MeSH Terms
Collagen / metabolism
Enzyme Inhibitors / pharmacology
Extracellular Matrix / metabolism
Fibroblasts / metabolism
Gingiva / metabolism
Gingivitis / enzymology*,  metabolism
Macrophages / metabolism
Matrix Metalloproteinase 14 / metabolism
Matrix Metalloproteinase 2 / metabolism
Matrix Metalloproteinase 9 / metabolism
Matrix Metalloproteinases / biosynthesis*,  chemistry
Neutrophils / metabolism
Rats, Wistar
Reg. No./Substance:
0/Enzyme Inhibitors; 9007-34-5/Collagen; EC 3.4.24.-/Matrix Metalloproteinases; EC Metalloproteinase 2; EC Metalloproteinase 9; EC Metalloproteinase 14

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