| Changes in MMPs and inflammatory cells in experimental gingivitis. | |
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MedLine Citation:
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PMID: 19085832 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In periodontal disease, extensive disorganization of the extracellular matrix promotes the loss of adhesion between the teeth and periodontium. A previous study suggested a reduction in the area occupied by collagen in the gingiva, during the first week of periodontal disease induction, however, the remaining fibers were more compact and thicker. Therefore, it was decided to investigate which of the MMP-2, -9, -14 and RECK, an MMP inhibitor, were involved in these modifications taking place in early gingivitis induced by ligature. The results of gene expression analysis indicated no changes for RECK. MMP-14 showed a reduction at 7 days of inflammation, and there was an immediate increase in MMP-2 gene expression and enzymatic activity, apparently by the stimulation of resident cells such as fibroblasts. A peak of MMP-9 expression 5 days after ligature followed after the peak of enzymatic activity found two days earlier. This pattern was consistent with the kinetics of macrophage and neutrophil recruitment. Immunohistochemistry suggested that MMP-9 was produced by both resident and inflammatory cells. Based on this evidence, it is suggested that extracellular matrix remodeling is related to MMP-2 and -9 production and activation. This allowed us to conclude that the host inflammatory response represents a significant factor for the advance of periodontal diseases. |
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Authors:
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Márcio Lorencini; Juliete A F Silva; Cristiane L R de la Hoz; Hernandes F Carvalho; Dagmar R Stach-Machado |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Histology and histopathology Volume: 24 ISSN: 1699-5848 ISO Abbreviation: Histol. Histopathol. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2008-12-16 Completed Date: 2009-02-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8609357 Medline TA: Histol Histopathol Country: Spain |
Other Details:
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Languages: eng Pagination: 157-66 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, State University of Campinas (UNICAMP), Campinas, SP, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Collagen / metabolism Enzyme Inhibitors / pharmacology Extracellular Matrix / metabolism Fibroblasts / metabolism Gingiva / metabolism Gingivitis / enzymology*, metabolism Inflammation* Macrophages / metabolism Male Matrix Metalloproteinase 14 / metabolism Matrix Metalloproteinase 2 / metabolism Matrix Metalloproteinase 9 / metabolism Matrix Metalloproteinases / biosynthesis*, chemistry Neutrophils / metabolism Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 9007-34-5/Collagen; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9; EC 3.4.24.80/Matrix Metalloproteinase 14 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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