Document Detail


Changes in ECG and enzyme activity in rat heart after myocardial infarction: effect of TPP and MnCl2.
MedLine Citation:
PMID:  19043979     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Heart infarction is one of the main causes of death in the human population. Assurance of a sufficient level of bioenergetic processes is very important for the heart after infarction. Mn2+ as well as thiamine pyrophosphate (TPP) are positive effectors of the pyruvate dehydrogenase complex (PDH) and the 2-oxoglutarate dehydrogenase complex (OGDH), both of which play a very important role in the Krebs cycle. Thus, we have established the effect of MnCl2 (10mg/kg) and TPP (20mg/kg)--4 injections every 12 h--on the activity of PDH, OGDH, lactate dehydrogenase (LDH) and malate dehydrogenase (MDH). Additionally, we perform an analysis of ECG to affirm the changes in the heart electrophysiology of healthy rats after MnCl2 and TPP treatment. We then analyzed changes in the activity of these enzymes after experimental myocardial infarction in rats. We observed a decrease of OGDH and MDH activity in rat hearts after infarction in comparison with sham-operated rats. Treatment of healthy rats with MnCl2 caused an increase of OGDH activity. Moreover both MnCl2 and TPP caused an increase of PDH activity and a decrease of MDH activity (TPP revealed a stronger effect). We found no changes in LDH activity. Electrocardiography data showed a slight shortening of the QT interval and an enhanced heartbeat rate after treatment with MnCl2. TPP caused only elongation of the QT interval. In conclusion, application of MnCl2 enhanced the activity of some very important enzymes in the respiration process (PDH and OGDH). This effect, connected with enhanced heartbeat and a slightly shortened ventricle relaxation, may have potential application during the key period of convalescence following heart infarction.
Authors:
A Tylicki; J Czerniecki; A Godlewska; M Kieliszek; T Zebrowski; T Bielawski; B Wojcik
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of physiology and biochemistry     Volume:  64     ISSN:  1138-7548     ISO Abbreviation:  J. Physiol. Biochem.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-12-02     Completed Date:  2008-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9812509     Medline TA:  J Physiol Biochem     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  93-101     Citation Subset:  IM    
Affiliation:
Department of Animal Biochemistry, Institute of Biology, University of Bialystok, Swierkowa 20B, 15-950 Bialystok, Poland. atyl@uwb.edu.pl
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MeSH Terms
Descriptor/Qualifier:
Animals
Chlorides / pharmacology*
Electrocardiography*
Heart* / drug effects,  physiopathology
Humans
Male
Manganese Compounds / pharmacology*
Myocardial Infarction* / enzymology,  physiopathology
Myocardium* / enzymology,  metabolism
Rats
Rats, Wistar
Thiamine Pyrophosphate / pharmacology*
Vitamin B Complex / pharmacology
Chemical
Reg. No./Substance:
0/Chlorides; 0/Manganese Compounds; 12001-76-2/Vitamin B Complex; 154-87-0/Thiamine Pyrophosphate; 7773-01-5/manganese chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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