| Changes in CLIP3 expression after sciatic nerve injury in adult rats. | |
| | |
MedLine Citation:
|
PMID: 23014974 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
CLIP3 (cytoplasmic linker protein 3) is a 547 amino acid residue cytoplasmic protein that localises to Golgi stacks and tubulovesicular elements juxtaposed to Golgi cisternae. Composed of three Ank (ankyrin) repeats and two CAP-Gly (cytoskeleton-associated protein-glycine) domains, CLIP3 may function as a cytoplasmic linker protein that is involved in TGN-endosome dynamics. To define the expression and role of CLIP3 during peripheral nervous system degeneration and regeneration, we created an acute sciatic nerve injury (SNI) model in adult rats. Western blot analyses revealed prominent up-regulation of CLIP3 and PCNA (proliferating cell nuclear antigen) protein levels at 3 days after SNI. Immunohistochemistry displayed that the expression of CLIP3 was noticeably increased in the injured nerve. Immunofluorescence further revealed that the CLIP3 and PCNA proteins colocalised respectively with S100 in the cytoplasm of Schwann cells. The expression profile of the SC/neuron co-cultures demonstrated that CLIP3 and PCNA protein levels were markedly expressed during the early stage of myelination. These results suggest that CLIP3 is likely associated with the myelination of proliferating Schwann cells, and nerve tissue regeneration after peripheral nerve injury. CLIP3 and PCNA expression during early myelination may be related to the direct uptake and transport of lipids and cholesterol, which were derived from the degenerating myelin, by Schwann cells to prepare for the formation of myelin sheath-like structures around regenerated axons after SNI. |
| | |
Authors:
|
Xiaolong Deng; Haixiang Wei; Dong Lou; Binbin Sun; Hailei Chen; Yi Zhang; Youhua Wang |
Related Documents
:
|
23259594 - Application of protein purification methods for the enrichment of a cytotoxin from camp... 3137684 - Effects of lipid-binding proteins apo a-i, apo a-il, beta 2-glycoprotein i, and c-react... 23091704 - Role of hsp90 in systemic lupus erythematosus and its clinical relevance. 23136524 - An atypical bhlh protein encoded by positive regulator of grain length 2 is involved in... 15683604 - Adipocytokines: emerging therapeutic targets. 12714064 - Functional profiling of the proteome with affinity labels. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-9-27 |
Journal Detail:
|
Title: Journal of molecular histology Volume: - ISSN: 1567-2387 ISO Abbreviation: J. Mol. Histol. Publication Date: 2012 Sep |
Date Detail:
|
Created Date: 2012-9-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101193653 Medline TA: J Mol Histol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Retinoic acid regulates commitment of undifferentiated mesenchymal stem cells into osteoblasts and a...
Next Document: Comparison of intracorporeal single-stapled and double-stapled anastomosis in laparoscopic low anter...