Document Detail


Changes in BRCA2 expression during progression of the cell cycle.
MedLine Citation:
PMID:  9168997     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been shown that genetic alterations in BRCA1 and BRCA2 can predispose an individual to develop breast cancer. We investigated the expression of both BRCA2 and BRCA1 during the progression of the cell cycle by northern blot analysis. In MCF-10F (normal breast epithelial cell line) and MCF-7 (breast cancer cell line) cells the expression of BRCA2 RNA was low in G0 and early G1 phases then up-regulated at the G1/S phase junction. Expression of BRCA2 was maintained at relatively high levels when cells progressed through S and G2/M phases. For MCF-7 cells, the level of BRCA2 transcript decreased as cells were released from nocodazole-mediated metaphase arrest. This is consistent with the observation of low but detectable BRCA2 RNA level in G1 phase of the cell cycle. For both cell lines, the patterns of RNA expression of BRCA1 and BRCA2 were similar during the proliferation phase of cell cycle. However, the transcripts from both genes were undetectable in quiescent cells. These results suggest important functions for both BRCA2 and BRCA1 in regulation of cell growth.
Authors:
S C Wang; S H Lin; L K Su; M C Hung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  234     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-06-24     Completed Date:  1997-06-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  247-51     Citation Subset:  IM    
Affiliation:
Department of Tumor Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
BRCA2 Protein
Blotting, Northern
Breast / cytology*,  metabolism
Breast Neoplasms / genetics,  pathology*
Cell Cycle*
Cell Line
Electrophoresis, Agar Gel
Flow Cytometry
Gene Expression*
Genes, BRCA1
Humans
Interphase
Mitosis
Neoplasm Proteins / genetics*
Nocodazole / pharmacology
RNA, Messenger / metabolism
S Phase
Transcription Factors / genetics*
Transcription, Genetic
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
CA16672/CA/NCI NIH HHS; R01-CA58880/CA/NCI NIH HHS; R01-CA60856/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/BRCA2 Protein; 0/Neoplasm Proteins; 0/RNA, Messenger; 0/Transcription Factors; 31430-18-9/Nocodazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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