| Changes in ATP level and iron-induced ultra-weak photon emission in bull spermatozoa, caused by membrane peroxidation during thermal stress. | |
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MedLine Citation:
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PMID: 9241365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ATP level, cell motility and viability, oxygen uptake, pyruvate kinase activity, and ultra-weak photon emission (UPE) induced by red-ox Fe(2+)-ascorbate cycling system were studied in fresh, in previously equilibrated in a glycerol diluent, and in cryopreserved bull spermatozoa, exposed to thermal stress by incubation of the cells at 44 degrees C. A sharp drop in motility and viability of fresh spermatozoa and even more so, of equilibrated and cryopreserved cells was accompanied by accumulation of ATP. When cell movement was totally inhibited, ATP utilization was decreased, while chemical energy continued to be produced by cell pyruvate kinase, one of the key glycolytic enzymes, which in spermatozoa is very active (6500 IU/g protein) and insensitive to feed-back inhibition by excess of ATP and L-cysteine. Accumulation of ATP during incubation at 44 degrees C in 0.9% NaCl was accompanied by rapid decrease in oxygen consumption by fresh spermatozoa and an increase in Fe(2+)-ascorbate induced UPE, followed by a sharp decrease in ATP level observed at the end of induced UPE measurement. The increase in photon emission due to lipid peroxidation was highly correlated with the increase in cell ATP level caused by thermal stress. |
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Authors:
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M Gumińska; T Kedryna; A Laszczka; M Godlewski; J Sławiński; B Szcześniak-Fabiaņczyk; T Kwiecińska; Z Rajfur; D Wierzuchowska |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta biochimica Polonica Volume: 44 ISSN: 0001-527X ISO Abbreviation: Acta Biochim. Pol. Publication Date: 1997 |
Date Detail:
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Created Date: 1997-09-09 Completed Date: 1997-09-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 14520300R Medline TA: Acta Biochim Pol Country: POLAND |
Other Details:
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Languages: eng Pagination: 131-8 Citation Subset: IM |
Affiliation:
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Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Cracow, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism* Animals Cattle Cell Membrane / metabolism* Heat Stress Disorders* Iron / pharmacology* Male Oxidation-Reduction Photons Spermatozoa / metabolism* |
| Chemical | |
Reg. No./Substance:
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56-65-5/Adenosine Triphosphate; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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