| Changes of heavy metal, metallothionein and heat shock proteins in Sertoli cells induced by cadmium exposure. | |
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MedLine Citation:
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PMID: 18556172 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, we examined the levels of Cadmium (Cd), iron (Fe) and zinc (Zn), which were considered to be involved in Sertoli cell damage caused by Cd exposure. We also examined metallothionein (MT), heat shock protein 70 (Hsp70) and heme oxygenase-1 (HO-1) expressions in Sertoli cells induced by Cd exposure. Evaluation by the in-air micro-particle induced X-ray emission (PIXE) method revealed that Cd and Fe distribution was increased in the cytoplasm of Sertoli cells after Cd exposure. By contrast, Zn was decreased in the cytoplasm of Sertoli cells after Cd exposure. It was suggested that the target of Cd toxicity was the cytoplasm of Sertoli cells, Fe was considered to enhance damage to Sertoli cells caused by Cd exposure. The DNA fragmentation rate was determined by ELISA after Cd exposure to Sertoli cells. It remained essentially unchanged with 2.5 microM Cd exposure of Sertoli cells; however, MT, Hsp70 and HO-1 were significantly increased by Cd exposure. As a result, Cd-induced MT was protected Sertoli cells against apoptosis, and Cd-induced HO-1 was involved in protection against oxidative stress. Incidentally, MT, Hsp70 and HO-1 showed similar responses to Cd exposure. |
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Authors:
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Takahiko Kusakabe; Katsuyuki Nakajima; Kyoumi Nakazato; Keiji Suzuki; Hisashi Takada; Takahiro Satoh; Masakazu Oikawa; Kazuo Arakawa; Takeaki Nagamine |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-10 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 22 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-18 Completed Date: 2008-10-21 Revised Date: 2009-04-10 |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 1469-75 Citation Subset: IM |
Affiliation:
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School of Health Sciences, Faculty of Medicine, Gunma University, 3-39-15 Shouwa-machi, Maebashi, Gunma, Japan. researcher.kusakabe@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects Cadmium / toxicity* Cytoplasm / drug effects, metabolism DNA Fragmentation / drug effects Enzyme-Linked Immunosorbent Assay Gene Expression Regulation / drug effects* HSP70 Heat-Shock Proteins / drug effects, metabolism Heme Oxygenase-1 / drug effects*, metabolism Iron / metabolism Male Metallothionein / drug effects*, metabolism Oxidative Stress / drug effects Rats Rats, Wistar Sertoli Cells / drug effects, metabolism Spectrometry, X-Ray Emission Zinc / metabolism |
| Chemical | |
Reg. No./Substance:
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0/HSP70 Heat-Shock Proteins; 7439-89-6/Iron; 7440-43-9/Cadmium; 7440-66-6/Zinc; 9038-94-2/Metallothionein; EC 1.14.99.3/Heme Oxygenase-1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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