Document Detail


Changes of cell proliferation and differentiation in the developing brain of mouse.
MedLine Citation:
PMID:  17592525     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the cell proliferation and differentiation in the developing brain of mouse. METHODS: C57/BL6 mice were divided into 3 groups at random. Bromodeoxyuridine (BrdU) was injected into the brains in different development periods once a day for 7 d. The brains were retrieved 4 weeks after the last BrdU injection. Immunohistochemical and immunofluorescent studies were carried out for detecting cell proliferation (BrdU) and cell differentiation (NeuN, APC, Iba1, and S100beta), respectively. RESULTS: The number of BrdU labeled cells decreased significantly with the development of the brain. Cell proliferation was prominent in the cortex and striatum. A small portion of BrdU and NeuN double labeled cells could be detected in the cortex at the early stage of development, and in the striatum and CA of the hippocampus in all groups. The majority of BrdU labeled cells were neuroglia, and the number of neuroglia cells decreased dramatically with brain maturation. Neurogenesis is the major cytogenesis in the dentate gyrus. CONCLUSION: These results demonstrated that cell proliferation, differentiation and survival were age and brain region related.
Authors:
Lin Qiu; Chang-Lian Zhu; Xiao-Yang Wang; Fa-Lin Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience bulletin     Volume:  23     ISSN:  1673-7067     ISO Abbreviation:  -     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-06-26     Completed Date:  2007-07-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101256850     Medline TA:  Neurosci Bull     Country:  China    
Other Details:
Languages:  eng     Pagination:  46-52     Citation Subset:  IM    
Affiliation:
Department of Anesthesia, Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. qiulin2005@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Brain / cytology,  growth & development*
Bromodeoxyuridine
Cell Count
Cell Differentiation / physiology*
Cell Proliferation*
Cerebral Cortex / cytology,  growth & development
Corpus Striatum / cytology,  growth & development
Fluorescent Antibody Technique
Hippocampus / cytology,  growth & development
Male
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins / metabolism
Neuroglia / cytology,  physiology*
Neurons / cytology,  physiology*
Nuclear Proteins / metabolism
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/NeuN protein, mouse; 0/Nuclear Proteins; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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