Document Detail

Changes of NF-kB, p53, Bcl-2 and caspase in apoptosis induced by JTE-522 in human gastric adenocarcinoma cell line AGS cells: role of reactive oxygen species.
MedLine Citation:
PMID:  12046064     Owner:  NLM     Status:  MEDLINE    
AIM: To identify whether JTE-522 can induce apoptosis in AGS cells and ROS also involved in the process, and to investigate the changes in NF-kB, p53, bcl-2 and caspase in the apoptosis process. METHODS: Cell culture, MTT, Electromicroscopy, agarose gel electrophoresis, lucigenin, Western blot and electrophoretic mobility shift assay (EMSA) analysis were employed to investigate the effect of JTE-522 on cell proliferation and apoptosis in AGS cells and related molecular mechanisms. RESULTS: JTE-522 inhibited the growth of AGS cells and induced the apoptosis. Lucigenin assay showed the generation of ROS in cells under incubation with JTE-522. The increased ROS generation might contribute to the induction of AGS cells to apoptosis. EMSA and Western blot revealed that NF-kB activity was almost completely inhibited by preventing the degradation of IkBalpha. Additionally, by using Western blot we confirmed that the level of bcl-2 was decreased, whereas p53 showed a great increase following JTE-522 treatment. Their changes were in a dose-dependent manner. CONCLUSION: These findings suggest that reactive oxygen species, NF-kB, p53, bcl-2 and caspase-3 may play an important role in the induction of apoptosis in AGS cells after treatment with JTE-522.
Hong-Liang Li; Dan-Dan Chen; Xiao-Hong Li; Hai-Wei Zhang; Yan-Qing Lu; Chun-Ling Ye; Xian-Da Ren
Related Documents :
19483104 - Sorafenib and vorinostat kill colon cancer cells by cd95-dependent and -independent mec...
17286744 - Inhibition of proliferation and induction of apoptosis by melatonin in human myeloid hl...
21517994 - Origin of neointimal cells in arteriovenous fistulae: bone marrow, artery, or the vein ...
12046064 - Changes of nf-kb, p53, bcl-2 and caspase in apoptosis induced by jte-522 in human gastr...
20885774 - A mathematical model of β-cells in an islet of langerhans sensing a glucose gradient.
3969444 - Effect of confluent holding on potentially lethal damage repair, cell cycle progression...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  8     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-04     Completed Date:  2002-08-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  431-5     Citation Subset:  IM    
Department of Pharmacology, Jinan University Pharmacy College, Guangzhou 510632, Guangdong, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenocarcinoma / metabolism*,  pathology
Apoptosis / drug effects
Benzenesulfonates / pharmacology
Caspases / metabolism
Cell Division / drug effects
DNA-Binding Proteins / metabolism
I-kappa B Proteins*
NF-kappa B / metabolism
Oxazoles / pharmacology
Proto-Oncogene Proteins c-bcl-2 / metabolism
Reactive Oxygen Species / metabolism
Stomach Neoplasms / metabolism*,  pathology
Tumor Cells, Cultured
Tumor Suppressor Protein p53 / metabolism
Reg. No./Substance:
0/4-(4-cyclohexyl-2-methyloxazol-5-yl)-2-fluorobenzenesulfonamide; 0/Benzenesulfonates; 0/DNA-Binding Proteins; 0/I-kappa B Proteins; 0/NF-kappa B; 0/Oxazoles; 0/Proto-Oncogene Proteins c-bcl-2; 0/Reactive Oxygen Species; 0/Tumor Suppressor Protein p53; 139874-52-5/NF-kappaB inhibitor alpha; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Rapid screening mitochondrial DNA mutation by using denaturing high-performance liquid chromatograph...
Next Document:  Inhibition of human telomerase in MKN-45 cell line by antisense hTR expression vector induces cell a...