| Changed mitochondrial function by pre- and/or postpartum diet alterations in sheep. | |
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MedLine Citation:
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PMID: 19826104 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In a sheep model, we investigated diet effects on skeletal muscle mitochondria to look for fetal programming. During pregnancy, ewes were fed normally (N) or were 50% food restricted (L) during the last trimester, and lambs born to these ewes received a normal (N) or a high-fat diet (H) for the first 6 mo of life. We examined mitochondrial function in permeabilized muscle fibers from the lambs at 6 mo of age (adolescence) and after 24 mo of age (adulthood). The postpartum H diet for the lambs induced an approximately 30% increase (P < 0.05) of mitochondrial VO(2max) and an approximately 50% increase (P < 0.05) of the respiratory coupling ratio (RCR) combined with lower levels of UCP3 and PGC-1alpha mRNA levels (P < 0.05). These effects proved to be reversible by a normal diet from 6 to 24 mo of age. However, at 24 mo, a long-term effect of the maternal gestational diet restriction (fetal programming) became evident as a lower VO(2max) (approximately 40%, P < 0.05), a lower state 4 respiration (approximately 40%, P < 0.05), and lower RCR ( approximately 15%, P < 0.05). Both PGC-1alpha and UCP3 mRNA levels were increased (P < 0.05). Two analyzed muscles were affected differently, and muscle rich in type I fibers was more susceptible to fetal programming. We conclude that fetal programming, seen as a reduced VO(2max) in adulthood, results from gestational undernutrition. Postnatal high-fat diet results in a pronounced RCR and VO(2max) increase in adolescence. However, these effects are reversible by diet correction and are not maintained in adulthood. |
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Authors:
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Wenche J??rgensen; Christiane Gam; Jesper L??vind Andersen; Peter Schjerling; Morten Scheibye-Knudsen; Ole Hartvig Mortensen; Niels Grunnet; Mette Olaf Nielsen; Bj??rn Quistorff |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-13 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 297 ISSN: 1522-1555 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-25 Completed Date: 2009-12-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E1349-57 Citation Subset: IM |
Affiliation:
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Department of Biomedical Sciences, Nuclear Magnetic Resonance Center, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark. wenche@sund.ku.dk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Biopsy DNA, Mitochondrial / chemistry, genetics Female Fetal Development / physiology* Malnutrition / metabolism Maternal Nutritional Physiological Phenomena / physiology* Mitochondria, Muscle / metabolism, physiology* Muscle Fibers, Skeletal / metabolism, physiology Muscle, Skeletal / metabolism, physiology* Oxygen Consumption / physiology PPAR delta / genetics, metabolism Pregnancy RNA, Messenger / chemistry, genetics Reverse Transcriptase Polymerase Chain Reaction Sheep / physiology* Transcription Factors / genetics, physiology Uncoupling Agents / metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Mitochondrial; 0/PPAR delta; 0/RNA, Messenger; 0/Transcription Factors; 0/Uncoupling Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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