| Change of potassium current density in rabbit corneal epithelial cells during maturation and cellular senescence. | |
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MedLine Citation:
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PMID: 16440065 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Voltage-gated potassium (K+) channels may participate in cellular developmental regulation, including cell differentiation, proliferation and apoptosis. This study investigated the change of K + current densities in corneal epithelial cells during maturation and cellular senescence. METHODS: New Zealand white rabbits were divided into three age groups: newborn (<or= 7 days old, n = 18); young (8-12 weeks old, n = 59); and adult (20-28 weeks old, n = 16). Rabbit corneal epithelial cells were subdivided into the following three groups: small cells with capacitance < 6.0 pF; medium cells with capacitance 6.0-10.0 pF; and large cells with capacitance > 10.0 pF. Using a whole-cell clamp technique, K+ current was recorded and current densities were calculated. Differences in K+ current densities among newborn, young and adult rabbits, as well as differences among small, medium and large cells, were analyzed. RESULTS: We delineated two types of cells manifesting different amplitudes of depolarization-activated K+ outward currents. The averaged current density of type 1 response cells was significantly larger than that of type 2 cells in newborn, young, and adult groups. For newborn epithelial cells, the depolarization-gated outward K+ current density decreased from small to medium to large cells (p = 0.049, at a membrane potential of 140 mV). A similar pattern of change in current density was also delineated for these cell sizes in young and adult rabbit corneal cells (p < 0.001 for both young and adult rabbits). An increase in depolarization-gated outward K+ current density was also delineated from newborn to young to adult rabbits (p < 0.001, p < 0.001 and p < 0.006 for small, medium and large cells, respectively, at a membrane potential of 140 mV). CONCLUSIONS: Corneal epithelial cells expressed K+ channel densities that were distinct from basal to superficial cells and from newborn to adult rabbits. |
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Authors:
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Shu-Wen Chang; Gwo-Jyh Chang; Ming-Jai Su |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the Formosan Medical Association = Taiwan yi zhi Volume: 105 ISSN: 0929-6646 ISO Abbreviation: J. Formos. Med. Assoc. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-01-27 Completed Date: 2006-02-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9214933 Medline TA: J Formos Med Assoc Country: China |
Other Details:
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Languages: eng Pagination: 7-16 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Far Eastern Memorial Hospital, Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan, R.O.C. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Aging / physiology* Epithelial Cells / metabolism* Epithelium, Corneal / cytology, metabolism* Potassium Channels, Voltage-Gated / metabolism* Rabbits |
| Chemical | |
Reg. No./Substance:
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0/Potassium Channels, Voltage-Gated |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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