| Change in the ileal bacterial population of turkeys fed different diets and after infection with Salmonella as determined with denaturing gradient gel electrophoresis of amplified 16s ribosomal DNA. | |
MedLine Citation:
|
PMID: 18577625 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Changes in ileal bacterial populations of Salmonella-infected turkeys fed different diets were analyzed by using 16S-V3 PCR denaturing gradient gel electrophoresis. Turkeys raised on litter flooring were fed wheat- and corn-based diets with and without enzyme preparations (XY1 and XY2, respectively) from 0 to 126 d. Preparation XY1 contained exclusively endoxylanase, whereas preparation XY2 contained endoxylanase, protease, and alpha-amylase (Danisco, , Wiltshire, UK). The dietary activity levels of XY1 and XY2 were 2,500 and 650 endo-1,4-beta-xylanase units/kg of feed, respectively. Microbial DNA was extracted from the ileal content of 16-wk-old turkeys, and the 16S rDNA gene was amplified by PCR and analyzed by denaturing gradient gel electrophoresis. Diversity indexes, including richness (number of species, S), evenness (relative distribution of species, EH), diversity (using Shannon's index, H'), and Sorenson's pairwise similarities coefficient (measures the species in common between different habitats, Cs) were calculated. Additionally, diversity indexes were associated with Salmonella prevalence determined from fresh fecal droppings collected from each pen. On the basis of contrast analysis, the wheat-based diets resulted in higher microbial diversity indexes than the corn-based diets (S = 10 vs. 12; EH = 0.9 vs. 0.8; H' = 2.2 vs. 1.9, P < 0.05). Likewise, enzyme supplementation stimulated growth of the microbiota and increased the diversity indexes in comparison with unsupplemented treatments (S = 13 vs. 10; EH = 0.9 vs. 0.8; H' = 2.2 vs. 1.9, P < 0.05). Salmonella prevalence was higher (P < 0.05) at 15 wk in turkeys fed the corn-based diet (Salmonella prevalence = 50%) than in turkeys fed the corn-enzyme (Salmonella prevalence = 13%) and wheat-based (Salmonella prevalence = 0%) dietary treatments. Therefore, contrast analysis showed that birds fed the corn control diet had lower microbiota diversity but higher Salmonella prevalence than birds fed the enzyme-supplemented and wheat-based diets. In contrast, birds fed the wheat-based diets had higher diversity but lower Salmonella prevalence than birds fed the corn-based diets. High dietary nonstarch polysaccharides from wheat and dietary exogenous enzyme supplementation promoted microbial community diversity and apparently discouraged Salmonella colonization through competitive exclusion. Nonstarch polysaccharides and dietary exogenous enzyme supplementation may be practical tools to control enteric pathogens and benefit the intestinal health and food safety of the birds. |
Authors:
|
A A Santos; P R Ferket; F B O Santos; N Nakamura; C Collier |
Related Documents
:
|
20680875 - Effects of different yeast cell wall supplements added to maize- or wheat-based diets f... 6721805 - The influence of nutrient density of broiler rations on the effective dose of anticocci... 8677425 - Efficacy of maduramicin against turkey coccidiosis in battery: a clinical and pathologi... 8101165 - Liver stearyl-coa desaturase activity and fatness in birds. in vitro studies in the gro... 15217005 - Fat digestibility in equus caballus follows increasing first-order kinetics. 19375595 - Very low-density lipoprotein in the cord blood of preterm neonates. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Poultry science Volume: 87 ISSN: 0032-5791 ISO Abbreviation: Poult. Sci. Publication Date: 2008 Jul |
Date Detail:
|
Created Date: 2008-06-25 Completed Date: 2008-10-28 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0401150 Medline TA: Poult Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 1415-27 Citation Subset: IM |
Affiliation:
|
Department of Health and Biomedical Sciences, Florida Hospital College of Health Sciences, Orlando, FL 32803, USA. anael.santos@fhchs.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animal Feed
/
analysis Animal Nutritional Physiological Phenomena Animals Diet / veterinary* Dietary Supplements Electrophoresis, Gel, Two-Dimensional Gastrointestinal Contents / microbiology Ileum / microbiology* Nucleic Acid Denaturation Polymerase Chain Reaction / veterinary RNA, Ribosomal, 16S / genetics* Salmonella Infections / microbiology* Triticum Turkeys / microbiology* Zea mays |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interactive effects of ammonia and light intensity on hematochemical variables in broiler chickens.
Next Document: Influxed insects as vectors for Campylobacter jejuni and Campylobacter coli in Danish broiler houses...