Document Detail

Change in gene expression subsequent to induction of Pnn/DRS/memA: increase in p21(cip1/waf1).
MedLine Citation:
PMID:  11494129     Owner:  NLM     Status:  MEDLINE    
Pnn (PNN) is a nuclear and cell adhesion-related protein. Previous work has suggested that Pnn/DRS/memA is a potential tumor suppressor involved in the regulation of cell adhesion and cell migration. Using the ecdysone-inducible mammalian expression system, a stable inducible GFP-tagged human Pnn gene (PNNGFP) expressing 293 cell line was created (EcR293-PNNGFP). Cells induced to express PNNGFP not only exhibited increased cell-cell adhesion but also exhibited changes in cell growth and cell cycle progression. cDNA array analyses, together with real time PCR, revealed that the effects of exogenously expressed Pnn on cellular behavior may be linked to the regulation of the expression of specific subset genes. This subset includes cell cycle-related genes such as p21(cip1/waf1), CDK4, CPR2; cell migration and invasion regulatory genes such as RhoA, CDK5, TIMP-1, MMP-7, and EMMPRIN; and MIC-1. Concordant with previous observations of Pnn-induced phenotype changes, genes coding for epithelial associated processes and cell division controls were elevated, while those coding for increased cell motility and cellular reorganizations were downregulated. We utilized p21 promoter-luciferase reporter constructs and demonstrated that a marked stimulation of p21 promoter activity in 293 cells correlated with increased Pnn expression. Taken together, these data indicate that Pnn may participate in the regulation of gene expression, thereby, positively promoting cell-cell adhesion, and negatively affecting cell migration and cell proliferation.
Y Shi; M N Simmons; T Seki; S P Oh; S P Sugrue
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  20     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-08-08     Completed Date:  2001-08-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  4007-18     Citation Subset:  IM    
Department of Anatomy and Cell Biology, University of Florida College of Medicine, Archer Road, Gainesville, Florida, FL 32610-0235, USA.
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MeSH Terms
Cell Adhesion / genetics
Cell Adhesion Molecules / biosynthesis,  genetics,  physiology*
Cell Cycle / genetics
Cell Cycle Proteins / biosynthesis,  genetics
Cell Division / genetics
Cell Line
Cell Movement / genetics
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / biosynthesis*,  genetics
DNA, Complementary / genetics
Ecdysone / pharmacology
Gene Expression Profiling
Gene Expression Regulation*
Genes, Reporter
Genes, Synthetic
Kidney / cytology
Luciferases / biosynthesis,  genetics
Nuclear Proteins / biosynthesis,  genetics,  physiology*
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Promoter Regions, Genetic
Recombinant Fusion Proteins / physiology
Transcription, Genetic / genetics
Grant Support
Reg. No./Substance:
0/CDKN1A protein, human; 0/Cell Adhesion Molecules; 0/Cell Cycle Proteins; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/DNA, Complementary; 0/Nuclear Proteins; 0/PNN protein, human; 0/Recombinant Fusion Proteins; 3604-87-3/Ecdysone; EC 1.13.12.-/Luciferases

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