Document Detail

Change of antioxidant enzymes activity of hazel (Corylus avellana L.) cells by AgNPs.
MedLine Citation:
PMID:  25404256     Owner:  NLM     Status:  Publisher    
Elicitation effect of silver nano particles (AgNPs) and triggering of defence system by production of hydrogen peroxide (H2O2) as a signaling molecule in the regulation of the activity of stress-related enzymes and production of Taxol was evaluated in suspension- cultured hazel cells (Corylus avellana L.). The cells were treated with different concentrations of AgNPs (0, 2.5, 5, and 10 ppm), in their logarithmic growth phase (d7) and were harvested after 1 week. Treatment of hazel cells with AgNPs decreased the viability of the cells. Also the results showed that while the activity of certain radical scavenging enzymes in particular of catalase and peroxidase increased by 2.5 and 5 ppm AgNPs, the activity of superoxide dismutase decreased in these treatments. The highest activity of ascorbate peroxidase was observed in 10 ppm AgNPs treatments. This treatment also showed the highest contents of H2O2 and phenolic compounds, as well as the highest activity of phenylalanine ammonialyase. According to the results, 5 ppm AgNPs was the best concentration for elicitation of hazel cells to produce efficient amounts of H2O2 in order for stimulation of antioxidant defence system, production of Taxol at the highest capacity of the cells, meanwhile reserving their viability.
Mitra Jamshidi; Faezeh Ghanati; Ayatollah Rezaei; Ebrahim Bemani
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-11-18
Journal Detail:
Title:  Cytotechnology     Volume:  -     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-11-18     Completed Date:  -     Revised Date:  2014-11-19    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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