| Chalcones and dihydrochalcones augment TRAIL-mediated apoptosis in prostate cancer cells. | |
| | |
MedLine Citation:
|
PMID: 20714300 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Chalcones and dihydrochalcones exhibit chemopreventive and antitumor activity. TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) is a natural endogenous anticancer agent. We examined the cytotoxic and apoptotic effect of chalcones and dihydrochalcones on TRAIL-mediated apoptosis in LNCaP prostate cancer cells. The cytotoxicity was evaluated by the MTT and LDH assays. The apoptosis was detected using annexin V-FITC by flow cytometry and fluorescence microscopy. The DeltaPsim was evaluated using DePsipher staining by fluorescence microscopy. Our study showed that two tested chalcones (chalcone and 2',6'dihydroxy-4'-methoxychalcone) and three dihydrochalcones (2',6'-dihydroxy-4'4-dimethoxydihydrochalcone, 2',6'-dihydroxy-4'-methoxydihydro- chalcone, and 2',4',6'-trihydroxydihydrochalcone, called phloretin) markedly augmented TRAIL-induced apoptosis and cytotoxicity in LNCaP cells and confirmed the significant role of chalcones in chemoprevention of prostate cancer. |
| | |
Authors:
|
Ewelina Szliszka; Zenon P Czuba; Bogdan Mazur; Andrzej Paradysz; Wojciech Krol |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-04 |
Journal Detail:
|
Title: Molecules (Basel, Switzerland) Volume: 15 ISSN: 1420-3049 ISO Abbreviation: Molecules Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-08-17 Completed Date: 2010-12-03 Revised Date: 2010-12-15 |
Medline Journal Info:
|
Nlm Unique ID: 100964009 Medline TA: Molecules Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 5336-53 Citation Subset: IM |
Affiliation:
|
Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana 19, Zabrze, Poland. eszliszka@sum.edu.pl |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects* Cell Line, Tumor Chalcones / chemistry, pharmacology* Drug Synergism Humans Male Membrane Potential, Mitochondrial / drug effects Prostatic Neoplasms / pathology* TNF-Related Apoptosis-Inducing Ligand / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Chalcones; 0/TNF-Related Apoptosis-Inducing Ligand; 1083-30-3/dihydrochalcone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Solid-phase synthesis of N-substituted glycine oligomers (alpha-peptoids) and derivatives.
Next Document: Effects of time point measurement on the reconstruction of gene regulatory networks.