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ChIP-on-chip to Identify Mutant p53 Targets.
MedLine Citation:
PMID:  23150450     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Chromatin immunoprecipitation (ChIP) followed by microarray hybridization (on-chip) is a technique well suited for a comprehensive analysis of transcription factor binding sites, histone modification patterns, and nucleosome occupancy. It can be restricted to a subset of genes or regions but also expanded up to a genome-wide range yielding insight into the functional elements of gene regulatory networks. Mutant p53 proteins have lost their capacity to bind to its cognate binding sites, but it is well established that it has retained the ability to bind indirectly to DNA via other transcription factors and therefore change the expression of several target genes. The identification of those transcription factors and binding regions sheds light on how mutant p53 is able to exert oncogenic functions.
Authors:
Frauke Goeman; Giulia Fontemaggi; Giovanni Blandino
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  962     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  211-226     Citation Subset:  -    
Affiliation:
Laboratory of Translational Oncogenomics, Regina Elena Cancer Institute, Rome, Italy.
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