| ChIP Sequencing to Identify p53 Targets. | |
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MedLine Citation:
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PMID: 23150451 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Mutant p53 may activate target genes through the interaction of transcription factors or through histone modifications. Chromatin immunoprecipitation (ChIP) is a method commonly used to study these types of protein interactions. In order to generate a list of target genes that may be activated through this mechanism, ChIP sequencing may be used. ChIP sequencing involves the mass parallel sequencing of ChIP DNA fragments. We describe a method by which to prepare chromatin immunoprecipitation sequencing libraries and how to analyze sequencing data. In this procedure, prepared libraries have been sent to a core facility. The results have been verified using quantitative PCR. |
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Authors:
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Catherine Vaughan; Brad Windle; Sumitra Deb |
Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 962 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: - |
Other Details:
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Languages: ENG Pagination: 227-236 Citation Subset: - |
Affiliation:
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Department of Integrative Life Sciences, Virginia Commonwealth University, Richmond, VA, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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