Document Detail


ChIP for Identification of p53 Responsive DNA Promoters.
MedLine Citation:
PMID:  23150449     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Chromatin immunoprecipitation assay (ChIP) has been frequently used to determine whether a transcriptional regulator can bind to a specific DNA element in the chromatin content of cells. Here, we describe a detailed protocol for this assay with hands-on tips based on our own experience in working on the transcriptional regulator and tumor suppressor p53.
Authors:
Jun-Ming Liao; Hua Lu
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  962     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  201-210     Citation Subset:  -    
Affiliation:
Department of Biochemistry & Molecular Biology and Simon Cancer Center, Indiana University School of Medicine, Indianapolis, IN, USA.
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