Document Detail

Cgr11 encodes a secretory protein involved in cell adhesion.
MedLine Citation:
PMID:  19473726     Owner:  NLM     Status:  MEDLINE    
We performed comparative proteomic analyses of pituitary tumor-derived cell lines, and found a new protein, preliminarily called hydrophobestin, which was produced only in somatotrophic cells, MtT/S, but not in non-hormone-producing cells, MtT/E. Hydrophobestin is encoded by the cell growth regulatory gene, Cgr11, which is known to have growth-suppressive potential in several cell lines. We have now sought to investigate the underlying events responsible for cell growth inhibition by hydrophobestin. Immunocytochemisty revealed that hydrophobestin is localized in the Golgi apparatus of MtT/S cells and Cgr11-transfected MtT/E cells. The apparent molecular mass of the protein was determined by Westerm blot analysis of conditioned culture medium of MtT/S cells. Our data show that hydrophobestin is a secretory protein localized in the pituitary gland, adrenal gland, digestive tract, reproductive organs, and kidney. We also found that hydrophobestin promotes compact monolayer cell aggregates in PC12 cells transfected with Cgr11, however, non-transfected, vector- or EF-hand motif-deleted (DeltaEF) Cgr11-transfected PC12 cells cannot form compact cell colonies. An antibody recognizing EF-hand motifs showed strong staining in the intercellular space of both Cgr11-transfected PC12 cells and MtT/S cells (Cgr11-expressing cells). Our data suggest that hydrophobestin-mediated cell adhesion may regulate cell growth through compact cell attachment.
Sukumar Devnath; Taro Kataoka; Kaoru Miura; Mie Kusuda; Kunio Kitamura; Yuko Kumada; Akikazu Mochiduki; Kei Kaneko; Akihito Adachi; Kinji Inoue
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-27
Journal Detail:
Title:  European journal of cell biology     Volume:  88     ISSN:  1618-1298     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-07-20     Completed Date:  2009-12-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  521-9     Citation Subset:  IM    
Department of Regulatory Biology, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama, Japan.
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MeSH Terms
Cell Adhesion*
Cell Adhesion Molecules / genetics*,  metabolism
Cell Line
Fluorescent Antibody Technique
Gene Expression
PC12 Cells
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Cgr11 protein, rat

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