| Cervical stroma apoptosis in pregnancy. | |
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MedLine Citation:
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PMID: 11239645 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine whether the percentage of apoptotic nuclei is different in cervical stroma of pregnant laboring women compared with nonpregnant women and pregnant nonlaboring women. METHODS: We took cervical stromal biopsies during cesarean delivery at the level of the lower uterine segment from ten women in active labor and 13 women before labor. In addition, we took biopsies of cervical stroma at the level of the internal cervical os from hysterectomy specimens in ten reproductive-aged women. Cryosections were then analyzed using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling staining. Tissue specimens were analyzed with ligation-mediated polymerase chain reaction to visualize nucleosomal ladders characteristic of apoptosis. To detect a 10% difference in the percentage of apoptotic cells per subject between study groups assuming a power of 0.90, an alpha of.05 in approximately ten subjects per group was needed. RESULTS: The median percentage of apoptotic nuclei was 0.7 (interquartile range 0.4, 1.4) for the nonpregnant group, 7.5 (interquartile range 6.6, 11.2) for the pregnant nonlaboring group, and 11.6 (interquartile range 8.3, 16.7) for the pregnant laboring group (P <.001). The percentage of apoptotic nuclei differed significantly across the three study groups. Using ligation-mediated polymerase chain reaction, nucleosomal ladders were seen in the specimens from pregnant women but not in the specimens from nonpregnant women, confirming the increase in stromal apoptosis seen with pregnancy. CONCLUSION: Apoptosis of cervical stromal cells may play a role in the remodeling of the cervix during pregnancy and contribute to cervical changes during labor. |
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Authors:
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A D Allaire; N D'Andrea; P Truong; M J McMahon; B A Lessey |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Obstetrics and gynecology Volume: 97 ISSN: 0029-7844 ISO Abbreviation: Obstet Gynecol Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-03-12 Completed Date: 2001-04-05 Revised Date: 2009-10-26 |
Medline Journal Info:
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Nlm Unique ID: 0401101 Medline TA: Obstet Gynecol Country: United States |
Other Details:
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Languages: eng Pagination: 399-403 Citation Subset: AIM; IM |
Affiliation:
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Divisions of Maternal-Fetal Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Apoptosis* Case-Control Studies Cervix Uteri / cytology*, physiology* Female Humans In Situ Nick-End Labeling Labor, Obstetric* Polymerase Chain Reaction Pregnancy* Stromal Cells / cytology |
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