Document Detail


Cervical ripening and insufficiency: from biochemical and molecular studies to in vivo clinical examination.
MedLine Citation:
PMID:  19303692     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To understand cervical ripening and especially the pathophysiology of cervical insufficiency, it is important to know the cervical composition: the cervix is dominated by fibrous connective tissue, consisting predominantly of Type I collagen (70%). Despite many studies of the cervix, we still rely upon relatively crude methods for clinical evaluation of the cervix. If the amount of cervical collagen plays a role in cervical insufficiency and in success of or length of induction of labor, then measurements of cervical collagen may provide an objective means of establishing the diagnosis or prognosis. We have established and reported a non-invasive means, called Collascope, to measure collagen cross-linking using light-induced fluorescence (LIF), and which is specifically designed to assess cervical ripening, and functions by measuring the natural fluorescence of non-soluble collagen in the cervix. Studies conducted in animals and humans in a variety of settings indicate that cervical function can be successfully monitored using the Collascope during pregnancy: LIF correlates negatively with gestational age and positively with time-to-delivery interval, and is predictive of delivery within 24h. Additionally LIF is significantly lower in women with cervical insufficiency. We suggest that the Collascope might be useful to better define management in cases of spontaneous preterm or induced term cervical ripening. From our studies and others, it is clear that in forecasting (pre-)term cervical ripening, the capability of the technologies and bioassays that have been generally accepted into clinical practice are limited. Any devices shown to be superior to the clinically accepted tests currently used should be quite useful for clinicians. The Collascope offers an objective measurement of both the function and state of the cervix, by directly measuring collagen cross-linking using LIF.
Authors:
Dietmar Schlembach; Lynette Mackay; Leili Shi; William L Maner; Robert E Garfield; Holger Maul
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-03-20
Journal Detail:
Title:  European journal of obstetrics, gynecology, and reproductive biology     Volume:  144 Suppl 1     ISSN:  1872-7654     ISO Abbreviation:  Eur. J. Obstet. Gynecol. Reprod. Biol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-10     Completed Date:  2009-07-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375672     Medline TA:  Eur J Obstet Gynecol Reprod Biol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  S70-6     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Medical University of Graz, Graz, Austria. drschlembach@gmx.net
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MeSH Terms
Descriptor/Qualifier:
Animals
Cervical Ripening / physiology*
Cervix Uteri / chemistry*,  drug effects,  physiology
Collagen Type I / analysis*
Diagnostic Techniques, Obstetrical and Gynecological*
Female
Fluorescence*
Humans
Pregnancy
Premature Birth / diagnosis,  prevention & control
Prostaglandins / therapeutic use
Spectrometry, Fluorescence / instrumentation
Uterine Cervical Incompetence / diagnosis
Grant Support
ID/Acronym/Agency:
7R01HD037480/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Collagen Type I; 0/Prostaglandins

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