| Cervical softening during pregnancy: regulated changes in collagen cross-linking and composition of matricellular proteins in the mouse. | |
| | |
MedLine Citation:
|
PMID: 21248285 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A greater understanding of the parturition process is essential in the prevention of preterm birth, which occurs in 12.7% of infants born in the United States annually. Cervical remodeling is a critical component of this process. Beginning early in pregnancy, remodeling requires cumulative, progressive changes in the cervical extracellular matrix (ECM) that result in reorganization of collagen fibril structure with a gradual loss of tensile strength. In the current study, we undertook a detailed biochemical analysis of factors in the cervix that modulate collagen structure during early mouse pregnancy, including expression of proteins involved in processing of procollagen, assembly of collagen fibrils, cross-link formation, and deposition of collagen in the ECM. Changes in these factors correlated with changes in the types of collagen cross-links formed and packing of collagen fibrils as measured by electron microscopy. Early in pregnancy there is a decline in expression of two matricellular proteins, thrombospondin 2 and tenascin C, as well as a decline in expression of lysyl hydroxylase, which is involved in cross-link formation. These changes are accompanied by a decline in both HP and LP cross-links by gestation Days 12 and 14, respectively, as well as a progressive increase in collagen fibril diameter. In contrast, collagen abundance remains constant over the course of pregnancy. We conclude that early changes in tensile strength during cervical softening result in part from changes in the number and type of collagen cross-links and are associated with a decline in expression of two matricellular proteins thrombospondin 2 and tenascin C. |
| | |
Authors:
|
Meredith L Akins; Katherine Luby-Phelps; Ruud A Bank; Mala Mahendroo |
Related Documents
:
|
17211995 - Efficacy and safety of etanercept, high-dose intravenous gammaglobulin and plasmapheres... 2657895 - Psychologic aspects of pregnancy in patients with rheumatic diseases. 7993705 - Clinical features of systemic lupus erythematosus. 10977895 - Routine sampling and the control of legionella spp. in cooling tower water systems. 20357175 - Individualized high-dose cabergoline therapy for hyperprolactinemic infertility in wome... 16015505 - Decitabine in chronic leukemias. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-01-19 |
Journal Detail:
|
Title: Biology of reproduction Volume: 84 ISSN: 1529-7268 ISO Abbreviation: Biol. Reprod. Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-04-21 Completed Date: 2011-08-23 Revised Date: 2012-09-19 |
Medline Journal Info:
|
Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
|
Languages: eng Pagination: 1053-62 Citation Subset: IM |
Affiliation:
|
Department of Obstetrics and Gynecology, The Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cervical Ripening / metabolism* Collagen / chemistry*, genetics, metabolism*, ultrastructure Extracellular Matrix Proteins / chemistry, metabolism*, ultrastructure Female Fibrillar Collagens / chemistry, genetics, metabolism, ultrastructure Gene Expression Regulation, Developmental Isoenzymes / genetics, metabolism Mice Mice, 129 Strain Mice, Inbred C57BL Microscopy, Electron, Transmission Pregnancy Pregnancy Proteins / chemistry*, genetics, metabolism*, ultrastructure Procollagen / chemistry, genetics, metabolism, ultrastructure Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / genetics, metabolism Protein Processing, Post-Translational RNA, Messenger / metabolism Tenascin / genetics, metabolism Thrombospondins / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
R01 HD043154/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Extracellular Matrix Proteins; 0/Fibrillar Collagens; 0/Isoenzymes; 0/Pregnancy Proteins; 0/Procollagen; 0/RNA, Messenger; 0/Tenascin; 0/Thrombospondins; 0/thrombospondin 2; 9007-34-5/Collagen; EC 1.14.11.4/Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Peroxiredoxin 2 Inhibits Granulosa Cell Apoptosis During Follicle Atresia Through the NFKB Pathway i...
Next Document: Resveratrol Modulates the Expression of PTGS2 and Cellular Proliferation in the Normal Rat Endometri...