| Cervical ripening and parturition in cows are driven by a cascade of pro-inflammatory cytokines. | |
| | |
MedLine Citation:
|
PMID: 19769639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The final stages of cervical ripening and parturition resemble an inflammatory process. Although the role of cytokines in both spontaneous and experimentally induced parturitions has been described in several small laboratory animals and humans, the involvement of pro-inflammatory and regulatory cytokines in physiologic parturition in cows has not been determined. In this study, the cytokine expression profiles were assessed in bovine cervical tissue at several stages of pregnancy and at parturition. Serial biopsy samples of the cervix were obtained from 10 cows on day 185 and day 275 of pregnancy (which was on average 5.4 days before parturition) and at parturition. Messenger RNA expression levels of interleukin (IL)-1beta, IL-6, IL-8, IL-10 and tumour necrosis factor (TNF)alpha were determined using real-time polymerase chain reaction and the number of neutrophils and eosinophils was estimated by Luna and Sirius Red staining. At parturition, IL-8 expression had increased 430-fold (p < 0.001) when compared with that of the day 185 of pregnancy, large numbers of neutrophils had invaded the cervix while eosinophils remained scarce, IL-1beta had increased eightfold (p < 0.05) and IL-6 had not changed significantly. Additionally, IL-10 was increased by 10-fold (p < 0.001) and TNFalpha decreased by 57% (p < 0.05) when compared with that of the day 185 of pregnancy. The large increase in expression of IL-8, enabling the influx of neutrophils, is indicative of its important role in the final stage of cervical ripening and at parturition. As previous studies have shown that neutrophils excrete matrix metalloproteinases (MMP), this might contribute to softening of the cervix. In contrast, the only slightly increased levels of IL-1, steady concentrations of IL-6 and decreased TNFalpha, the potential consequences of increased IL-10 expression, indicate that final cervical of cows in ripening at term parturition is an inflammatory process influenced by regulatory cytokines. |
| | |
Authors:
|
E van Engelen; M W de Groot; V N A Breeveld-Dwarkasing; M E Everts; G C van der Weyden; M A M Taverne; V P M G Rutten |
Related Documents
:
|
1442999 - Chemotactic factor in the pregnant rabbit uterine cervix. 7189159 - Maternal morbidity due to beta-adrenergic therapy. pre-existing cardiomyopathy aggravat... 8907339 - Development of corticospinal tract fibers and their plasticity i: quantitative analysis... 15622129 - The contribution of cervical propriospinal premotoneurons in recovering hemiparetic str... 7452159 - Termination of pregnancy by menstrual extraction. 1801679 - Intra-cervical prostaglandin e2 gel in management of dead fetus in utero. 8843809 - Increased fetal loss in women with heritable thrombophilia. 19151289 - Sepsis and acute renal failure in pregnancy. 11854649 - Parity and pregnancy outcomes. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Reproduction in domestic animals = Zuchthygiene Volume: 44 ISSN: 1439-0531 ISO Abbreviation: Reprod. Domest. Anim. Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-09-22 Completed Date: 2011-08-04 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 9015668 Medline TA: Reprod Domest Anim Country: Germany |
Other Details:
|
Languages: eng Pagination: 834-41 Citation Subset: IM |
Affiliation:
|
Department of Pathobiology, Division of Anatomy and Physiology, Utrecht University, Utrecht, The Netherlands. e.vanengelen@uu.nl |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cattle Cervical Ripening / physiology* Cervix Uteri / chemistry, cytology, physiology Cytokines / genetics, physiology* Female Gene Expression Gestational Age Inflammation Interleukin-10 / genetics Interleukin-1beta / genetics Interleukin-6 / genetics Interleukin-8 / genetics Leukocyte Count Neutrophils / physiology Parturition / physiology* Pregnancy RNA, Messenger / analysis Tumor Necrosis Factor-alpha / genetics |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; 0/Interleukin-1beta; 0/Interleukin-6; 0/Interleukin-8; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Dose-response studies for pituitary and testicular function in male dogs treated with the GnRH super...
Next Document: The long case and its modifications: a literature review.