Document Detail


Ceruloplasmin receptors in liver cell suspensions are limited to the endothelium.
MedLine Citation:
PMID:  6092118     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interaction of ceruloplasmin (CP) with isolated liver cell suspensions was studied using 125I-labeled and latex minibead-derivatized CP. Fractionation of liver cell suspensions was done using metrizamide gradient centrifugation. In crude liver cell suspensions only endothelial cells, but not hepatocytes and Kupffer cells bound the minibead probe. The binding was specific and inhibited by excess native CP. These results were confirmed using 125I-CP combined with cell fractionation technique. Kinetic data, obtained from the latter system, indicated a dissociation constant (Kd) of 1 X 10(-7) M and the number of receptors to be 5.7 X 10(5) per endothelial cell. The exclusive binding of CP to liver endothelium suggests that this cell may mediate the hepatocytes uptake of CP and is, therefore, a crucial element of the tissue-blood barrier.
Authors:
M Kataoka; M Tavassoli
Related Documents :
9588768 - Relationship between cellular shape and receptor-mediated endocytosis: an ultrastructur...
6840688 - Quantitation of hepatic granulomas and epithelioid cells in primary biliary cirrhosis.
6710808 - Pathology of naturally occurring chlamydiosis in african clawed frogs (xenopus laevis).
19653338 - Phenotypic changes of human cells in human-rat liver during partial hepatectomy-induced...
8835398 - Immunohistochemical analysis of the microanatomy of primate ovary.
7686158 - Direct demonstration of insulin-induced glut4 translocation to the surface of intact ce...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  155     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1984-12-07     Completed Date:  1984-12-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  232-40     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Ceruloplasmin / metabolism*
Endothelium / metabolism*
Liver / cytology,  enzymology,  metabolism*
Male
Rats
Rats, Inbred Strains
Receptors, Cell Surface / metabolism*
Grant Support
ID/Acronym/Agency:
AM-30142/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Cell Surface; EC 1.16.3.1/Ceruloplasmin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Differentiation-inducing agents decrease cryptic prolactin receptors in cultured rat mammary tumor c...
Next Document:  Mechanism of endonuclease banding of chromosomes.