Document Detail


Cerebrospinal fluid of newborn infants contains a deglycosylated form of the intermediate filament nestin.
MedLine Citation:
PMID:  8947955     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nestin is an intermediate filament protein found in CNS progenitor cells. Nestin reappears in CNS tumor cells and reactive astrocytes after CNS injury. In this study we investigated whether nestin could be detected in the cerebrospinal fluid (CSF) of newborn infants and whether expression levels change with gestational age (GA) and/or brain injury. Using Western blot analysis, we examined the expression of nestin in the CSF of newborn infants (GA 25-42 wk) with asphyxia (n = 14), periventricular leukomalacia and peri(intra)ventricular hemorrhage (n = 7), and in a control group (n = 11). Protein extract from the periventricular brain tissue of a 1-wk-old infant was also analyzed. Nestin was detected in all the CSF samples and in the protein extract from the periventricular brain tissue. Although the CSF levels of nestin expression did not change with increasing GA, the asphyxia group had significantly lower levels of nestin in the CSF. An unexpected finding was that brain-derived nestin had an apparent molecular mass of approximately 240 kD, whereas all analyzed CSF samples contained two nestin-immunoreactive proteins at 200 and 220 kD. Experimental deglycosylation of the 240-kD form reduced the molecular mass to 220 kD, indicating that nestin undergoes a specific deglycosylation upon release into the CSF.
Authors:
G Grigelioniené; M Blennow; C Török; G Fried; I Dahlin; U Lendahl; H Lagercrantz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pediatric research     Volume:  40     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-03-07     Completed Date:  1997-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  809-14     Citation Subset:  IM    
Affiliation:
Department of Woman and Child Health, Karolinska Institute, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Apgar Score
Asphyxia Neonatorum / cerebrospinal fluid*
Brain / metabolism
Cerebral Hemorrhage / cerebrospinal fluid*
Glycosylation
Humans
Infant, Newborn / cerebrospinal fluid*,  metabolism
Intermediate Filament Proteins / biosynthesis,  cerebrospinal fluid*
Leukomalacia, Periventricular / cerebrospinal fluid*
Nerve Tissue Proteins*
Polymerase Chain Reaction
Reference Values
Chemical
Reg. No./Substance:
0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/nestin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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