Document Detail


Cerebrospinal fluid miRNA profile in HIV-encephalitis.
MedLine Citation:
PMID:  23042033     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MicroRNAs are short non-coding RNAs that modulate gene expression by translational repression. Because of their high stability in intracellular as well as extracellular environments, miRNAs have recently emerged as important biomarkers in several human diseases. However, they have not been tested in the cerebrospinal fluid (CSF) of HIV-1 positive individuals. Here, we present results of a study aimed at determining the feasibility of detecting miRNAs in the CSF of HIV-infected individuals with and without encephalitis (HIVE). We also evaluated similarities and differences between CSF and brain tissue miRNAs in the same clinical setting. We utilized a high throughput approach of miRNA detection arrays and identified differentially expressed miRNAs in the frontal cortex of three cases each of HIV+, HIVE, and HIV- controls, and CSF of 10 HIV-positive and 10 HIV-negative individuals. For the CSF samples, the group of HIV+ individuals contained nine cases of HIV-associated neurological disorders (HAND) and, among those, four had HIVE. All the HIV-negative samples had non-viral acute disseminate encephalomyelitis. A total of 66 miRNAs were found differentially regulated in HIV+ compared to HIV- groups. The greatest difference in miRNA expression was observed when four cases of HIVE were compared to five non-HIVE cases, previously normalized with the HIV-negative group. After statistical analyses, 11 miRNAs were fund significantly up-regulated in HIVE. Although more clinical samples should be examined, this work represents the first report of CSF miRNAs in HIV-infection and offers the basis for future investigation.
Authors:
Marco Pacifici; Serena Delbue; Pasquale Ferrante; Duane Jeansonne; Ferdous Kadri; Steve Nelson; Cruz Velasco-Gonzalez; Jovanny Zabaleta; Francesca Peruzzi
Related Documents :
11211673 - Predictors of quality of life in aids patients.
16782673 - Social support and depressive symptomatology among hiv-positive women: the mediating ro...
8098183 - Correlation between cd4+ counts and prevalence of cytomegalovirus retinitis and human i...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  228     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-01-28     Completed Date:  2013-04-03     Revised Date:  2013-09-04    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1070-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
LSU Health Sciences Center, School of Medicine, Stanley S Scott Cancer Center, Louisiana State University, New Orleans, LA 70112, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cerebral Cortex / metabolism,  virology
Encephalitis* / cerebrospinal fluid,  complications,  pathology,  virology
Gene Expression Regulation
HIV Infections* / cerebrospinal fluid,  complications,  pathology,  virology
HIV-1 / isolation & purification
Humans
MicroRNAs* / cerebrospinal fluid,  genetics,  metabolism
Grant Support
ID/Acronym/Agency:
MH 079751/MH/NIMH NIH HHS; P60 AA009803-18/AA/NIAAA NIH HHS; R01 MH079751/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/MicroRNAs

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The invalidity of the photo-induced electron transfer mechanism for fluorescein derivatives.
Next Document:  Spastic cocontraction in hemiparesis: Effects of botulinum toxin.