Document Detail

Cerebrospinal fluid miRNA profile in HIV-encephalitis.
MedLine Citation:
PMID:  23042033     Owner:  NLM     Status:  MEDLINE    
MicroRNAs are short non-coding RNAs that modulate gene expression by translational repression. Because of their high stability in intracellular as well as extracellular environments, miRNAs have recently emerged as important biomarkers in several human diseases. However, they have not been tested in the cerebrospinal fluid (CSF) of HIV-1 positive individuals. Here, we present results of a study aimed at determining the feasibility of detecting miRNAs in the CSF of HIV-infected individuals with and without encephalitis (HIVE). We also evaluated similarities and differences between CSF and brain tissue miRNAs in the same clinical setting. We utilized a high throughput approach of miRNA detection arrays and identified differentially expressed miRNAs in the frontal cortex of three cases each of HIV+, HIVE, and HIV- controls, and CSF of 10 HIV-positive and 10 HIV-negative individuals. For the CSF samples, the group of HIV+ individuals contained nine cases of HIV-associated neurological disorders (HAND) and, among those, four had HIVE. All the HIV-negative samples had non-viral acute disseminate encephalomyelitis. A total of 66 miRNAs were found differentially regulated in HIV+ compared to HIV- groups. The greatest difference in miRNA expression was observed when four cases of HIVE were compared to five non-HIVE cases, previously normalized with the HIV-negative group. After statistical analyses, 11 miRNAs were fund significantly up-regulated in HIVE. Although more clinical samples should be examined, this work represents the first report of CSF miRNAs in HIV-infection and offers the basis for future investigation.
Marco Pacifici; Serena Delbue; Pasquale Ferrante; Duane Jeansonne; Ferdous Kadri; Steve Nelson; Cruz Velasco-Gonzalez; Jovanny Zabaleta; Francesca Peruzzi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  228     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-01-28     Completed Date:  2013-04-03     Revised Date:  2014-05-08    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1070-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Cerebral Cortex / metabolism,  virology
Encephalitis* / cerebrospinal fluid,  complications,  pathology,  virology
Gene Expression Regulation
HIV Infections* / cerebrospinal fluid,  complications,  pathology,  virology
HIV-1 / isolation & purification
MicroRNAs* / cerebrospinal fluid,  genetics,  metabolism
Grant Support
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