Document Detail


Cerebrospinal fluid B cells from multiple sclerosis patients are subject to normal germinal center selection.
MedLine Citation:
PMID:  17169437     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous findings from our laboratory demonstrated that some clonally expanded cerebrospinal fluid (CSF) B cells from MS patients exhibit diminished mutation targeting patterns in comparison to typical B cells selected in the context of germinal centers (GCs). In order to determine whether the overall CSF B cell repertoires adhered to mutation patterns typical of GC-selected B cells, we analyzed the immunoglobulin repertoires from CSF B cells of 8 MS patients for mutation characteristics typical of GC-derived B cells. Mutation targeting was preserved. Thus, clonal expansion of some CSF B cells may occur independently of GC, but the CSF B cell pool is governed by typical GC selection. Interestingly, the heavy chain CDR3's of CSF B cells from MS patients had a net acidic charge, similar to GC-derived B cells, but a tendency towards longer CDR3's, consistent with autoreactive B cells. How these findings may support current hypotheses regarding the origin of CSF B cells is discussed.
Authors:
Christopher Harp; Jane Lee; Doris Lambracht-Washington; Elizabeth Cameron; Gregory Olsen; Elliot Frohman; Michael Racke; Nancy Monson
Related Documents :
24576537 - Cyp26b1 promotes male germ cell differentiation by suppressing stra8-dependent meiotic ...
24093497 - Cell trivision of hyperploid cells.
22708077 - Scf(fbw7) modulates the nfkb signaling pathway by targeting nfkb2 for ubiquitination an...
24586247 - Loss of myoferlin redirects breast cancer cell motility towards collective migration.
10801447 - The drosophila bcl-2 family member dborg-1 functions in the apoptotic response to uv-ir...
24576537 - Cyp26b1 promotes male germ cell differentiation by suppressing stra8-dependent meiotic ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-12-13
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  183     ISSN:  0165-5728     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-12     Completed Date:  2007-04-05     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  189-99     Citation Subset:  IM    
Affiliation:
Department of Neurology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aged
B-Lymphocytes / immunology*
Female
Flow Cytometry / methods
Gene Rearrangement, B-Lymphocyte / genetics*
Genes, Immunoglobulin / genetics
Germinal Center / physiology*
Humans
Male
Middle Aged
Multiple Sclerosis / cerebrospinal fluid*,  genetics,  immunology*
Mutation
Nervous System Diseases / cerebrospinal fluid,  genetics,  immunology
Receptors, Antigen, B-Cell / immunology
Statistics, Nonparametric
Grant Support
ID/Acronym/Agency:
K24 NS 44250/NS/NINDS NIH HHS; R01 AI 47133/AI/NIAID NIH HHS; R01 NS 37513/NS/NINDS NIH HHS; R01 NS 40993/NS/NINDS NIH HHS; R01 NS040993-01A2/NS/NINDS NIH HHS; R01 NS040993-02/NS/NINDS NIH HHS; R01 NS040993-03/NS/NINDS NIH HHS; T32 AI 005284-28/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Antigen, B-Cell
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A preliminary study of cortisol and norepinephrine reactivity to psychosocial stress in borderline p...
Next Document:  Detection and typing of 46 genital human papillomaviruses by the L1F/L1R primer system based multipl...