| Cerebral tissue repair and atrophy after embolic stroke in rat: a magnetic resonance imaging study of erythropoietin therapy. | |
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MedLine Citation:
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PMID: 20722071 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using magnetic resonance imaging (MRI) protocols of T(2)-, T(2)*-, diffusion- and susceptibility-weighted imaging (T2WI, T2*WI, DWI, and SWI, respectively) with a 7T system, we tested the hypothesis that treatment of embolic stroke with erythropoietin (EPO) initiated at 24 hr and administered daily for 7 days after stroke onset has benefit in repairing ischemic cerebral tissue. Adult Wistar rats were subjected to embolic stroke by means of middle cerebral artery occlusion (MCAO) and were randomly assigned to a treatment (n = 11) or a control (n = 11) group. The treated group was given EPO intraperitoneally at a dose of 5,000 IU/kg daily for 7 days starting 24 hr after MCAO. Controls were given an equal volume of saline. MRI was performed at 24 hr and then weekly for 6 weeks. MRI and histological measurements were compared between groups. Serial T2WI demonstrated that expansion of the ipsilateral ventricle was significantly reduced in the EPO-treated rats. The volume ratio of ipsilateral parenchymal tissue relative to the contralateral hemisphere was significantly increased after EPO treatment compared with control animals, indicating that EPO significantly reduces atrophy of the ipsilateral hemisphere, although no significant differences in ischemic lesion volume were observed between the two groups. Angiogenesis and white matter remodeling were significantly increased and occurred earlier in EPO-treated animals than in the controls, as evident from T2*WI and diffusion anisotropy maps, respectively. These data indicate that EPO treatment initiated 24 hr poststroke promotes angiogenesis and axonal remodeling in the ischemic boundary, which may potentially reduce atrophy of the ipsilateral hemisphere. |
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Authors:
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Guangliang Ding; Quan Jiang; Lian Li; Li Zhang; Ying Wang; Zheng Gang Zhang; Mei Lu; Swayamprava Panda; Qingjiang Li; James R Ewing; Michael Chopp |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neuroscience research Volume: 88 ISSN: 1097-4547 ISO Abbreviation: J. Neurosci. Res. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-09-21 Completed Date: 2011-06-24 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: United States |
Other Details:
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Languages: eng Pagination: 3206-14 Citation Subset: IM |
Copyright Information:
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© 2010 Wiley-Liss, Inc. |
Affiliation:
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Department of Neurology, Henry Ford Hospital, Detroit, MI 48202, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Atrophy Disease Models, Animal Erythropoietin, Recombinant / pharmacology, therapeutic use* Intracranial Embolism / drug therapy*, etiology, pathology Magnetic Resonance Imaging / methods* Male Nerve Degeneration / drug therapy, etiology, pathology Neuroprotective Agents / pharmacology*, therapeutic use Random Allocation Rats Rats, Wistar Stroke / drug therapy*, etiology, pathology |
| Grant Support | |
ID/Acronym/Agency:
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HL64766/HL/NHLBI NIH HHS; P01 NS23393/NS/NINDS NIH HHS; P01 NS42345/NS/NINDS NIH HHS; R01 CA135329-03/CA/NCI NIH HHS; R01 NS048349-01A1/NS/NINDS NIH HHS; R01 NS048349-02/NS/NINDS NIH HHS; R01 NS048349-03/NS/NINDS NIH HHS; R01 NS048349-04/NS/NINDS NIH HHS; R01 NS048349-05/NS/NINDS NIH HHS; R01 NS062832-04/NS/NINDS NIH HHS; R01 NS064134-01A2/NS/NINDS NIH HHS; R01 NS064134-02/NS/NINDS NIH HHS; R01 NS48349/NS/NINDS NIH HHS; R01NS43324/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Erythropoietin, Recombinant; 0/Neuroprotective Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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