Document Detail

Cerebral phospholipid content and Na+,K+-ATPase activity during ischemia and postischemic reperfusion in the mongolian gerbil.
MedLine Citation:
PMID:  6330297     Owner:  NLM     Status:  MEDLINE    
Using bilateral carotid artery occlusion in adult gerbils we examined the effects of ischemia and ischemia/reperfusion on cerebral phospholipid content and Na+,K+-ATPase (EC activity. In contrast to the large changes in phospholipid content and membrane-bound enzyme activity that have been observed in liver and heart tissues, we observed relatively small changes in the cerebral content of total phospholipid, phosphatidylcholine (PC), phosphatidylserine (PS), and phosphatidylethanolamine (PE) following ischemic intervals of up to 240 min. Following 15 min of ischemia the cerebral content of sphingomyelin (SM) was decreased to less than 50% of control values but returned to near-normal levels with longer ischemic periods. Significant decreases in the cerebral content of phosphatidylinositol (PI) and phosphatidic acid (PA) were observed following shorter intervals of ischemia (15-45 min). Na+,K+-ATPase activity of cerebral homogenates prepared from the brains of gerbils subjected to 30-240 min of ischemia was decreased but significantly different from control activity only after 30 min of ischemia (-29%, p less than or equal to 0.05). With the exception of PS, reperfusion for 60 min following 60 min of ischemia resulted in marked increases in cerebral phospholipid content with PC, SM, PI, and PA levels exceeding and PE levels equal to preischemic values. Longer periods of reperfusion (180 min) resulted in decreases in cerebral phospholipid content toward (PC, SM, PI, and PA) or below (PE) preischemic levels. In contrast, the cerebral content of PS significantly decreased during reperfusion (-51% at 60 min, p less than or equal to 0.05) and remained below preischemic values even after 180 min of reperfusion.(ABSTRACT TRUNCATED AT 250 WORDS)
W H Enseleit; F R Domer; D M Jarrott; W H Baricos
Related Documents :
16029217 - Matrix metalloproteinase induction by emmprin in experimental focal cerebral ischemia.
11020767 - Periischemic cerebral blood flow (cbf) does not explain beneficial effects of isofluran...
18350127 - In vivo evaluation of the nitroimidazole-based thioflavin-t derivatives as cerebral isc...
8041137 - Neutrophil depletion attenuates human intestinal reperfusion injury.
25435837 - Analysis of the charge exchange between the human body and ground: evaluation of "earth...
15626577 - Influence of probenecid on the delivery of morphine-6-glucuronide to the brain.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  43     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1984 Aug 
Date Detail:
Created Date:  1984-08-08     Completed Date:  1984-08-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  320-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Brain / metabolism*
Ischemic Attack, Transient / metabolism*
Phosphatidic Acids / metabolism
Phosphatidylcholines / metabolism
Phosphatidylethanolamines / metabolism
Phosphatidylinositols / metabolism
Phosphatidylserines / metabolism
Phospholipids / metabolism*
Sodium-Potassium-Exchanging ATPase / metabolism*
Sphingomyelins / metabolism
Grant Support
5-T32-GM07177-05-0031/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Phosphatidic Acids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phosphatidylinositols; 0/Phosphatidylserines; 0/Phospholipids; 0/Sphingomyelins; EC ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Labeling of the electron entry site of cytochrome c oxidase using 51Cr2+.
Next Document:  Phase-shifting of cycles in level of serotonin N-acetyltransferase activity and cyclic GMP content o...