Document Detail


Cerebral metabolite dynamics during temporary complete ischemia in rats monitored by time-shared 1H and 31P NMR spectroscopy.
MedLine Citation:
PMID:  2319935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The changes in cerebral phosphorus metabolites, intracellular pH, and lactate during 30 min of complete global ischemia and 2 h of reperfusion were monitored by time-shared 1H and 31P in vivo NMR spectroscopy in rats. After the induction of ischemia, intracellular pH decreased from 7.14 +/- 0.01 to 6.32 +/- 0.10, and lactate concentration increased from 1.6 +/- 0.4 to 15.8 +/- 2.5 mumol/g; ATP and phosphocreatine were totally depleted, while inorganic phosphate increased 715 +/- 47%. Within 1 h after blood flow was restored, high-energy phosphates and lactate levels had recovered close to baseline levels. The changes in intracellular pH and lactate levels during ischemia and reperfusion correlated well.
Authors:
L H Chang; R Shirane; P R Weinstein; T L James
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine     Volume:  13     ISSN:  0740-3194     ISO Abbreviation:  Magn Reson Med     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-05-10     Completed Date:  1990-05-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8505245     Medline TA:  Magn Reson Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6-13     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Chemistry, School of Medicine, University of California, San Francisco 94143.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / analysis
Animals
Brain / metabolism*
Brain Chemistry
Brain Ischemia / metabolism*
Hydrogen
Hydrogen-Ion Concentration
Lactates / analysis
Magnetic Resonance Spectroscopy*
Phosphocreatine / analysis
Phosphorus
Rats
Rats, Inbred Strains
Time Factors
Grant Support
ID/Acronym/Agency:
NS 22022-02/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Lactates; 1333-74-0/Hydrogen; 56-65-5/Adenosine Triphosphate; 67-07-2/Phosphocreatine; 7723-14-0/Phosphorus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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