Document Detail

Cerebral inflammatory response after fetal asphyxia and hyperoxic resuscitation in newborn sheep.
MedLine Citation:
PMID:  17519806     Owner:  NLM     Status:  MEDLINE    
Resuscitation with pure oxygen at birth after fetal asphyxia may aggravate brain damage by inducing pro-inflammation. The toll-like receptors (TLRs) may serve a pro-inflammatory role in hyperoxemia during ischemia-reperfusion. Sixteen near-term fetal sheep (132-136 d) were subjected to 10 min of cord occlusion, delivery and mechanical ventilation with 100% O2 (n = 8), or 21% O2 (n = 8) for 30 min followed by normoxemia for 90 min. Eight sheep fetuses were delivered immediately with inspired O2 targeted at normoxemia for 120 min (controls). Levels and distributions of mRNAs for IL-1beta, TNF-alpha, IL-12p40, IL-18, IL-6, IL-10, IFN-gamma, TLR-2, -3 and -4 in cerebral tissue at 2 h after birth were evaluated with real-time polymerase chain reaction (PCR) and in situ hybridization. Expressions of IL-1beta, IL-12p40, TLR-2, and TLR-4 were increased in cortex/subcortex after resuscitation with 100% O2 compared with 21% O2 (all p < 0.05) and to controls (all p < 0.05). Increased cellular expression of IL-1beta was localized to sub-meningeal cortical layers and to sub-cortical white matter. Hyperoxic resuscitation at birth following fetal asphyxia induces a cerebral pro-inflammatory response with an up-regulation of TLR-2 and -4. These may be early events leading to increased tissue damage after exposure to hyperoxemia at birth.
Tina Markus; Stefan Hansson; Isis Amer-Wåhlin; Lena Hellström-Westas; Ola Didrik Saugstad; David Ley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pediatric research     Volume:  62     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-21     Completed Date:  2007-09-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  71-7     Citation Subset:  IM    
Department of Pediatrics, Lund University, 221 85 Lund, Sweden.
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MeSH Terms
Animals, Newborn
Cerebral Cortex / cytology,  immunology*,  pathology
Cytokines / genetics,  immunology
Fetus / physiology*
Inflammation / metabolism*
Interleukin-12 Subunit p40 / genetics,  metabolism
Interleukin-1beta / genetics,  metabolism
Molecular Sequence Data
Sheep, Domestic
Toll-Like Receptors / genetics,  metabolism
Reg. No./Substance:
0/Cytokines; 0/Interleukin-12 Subunit p40; 0/Interleukin-1beta; 0/Toll-Like Receptors

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