| Cerebral inflammatory response after fetal asphyxia and hyperoxic resuscitation in newborn sheep. | |
| | |
MedLine Citation:
|
PMID: 17519806 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Resuscitation with pure oxygen at birth after fetal asphyxia may aggravate brain damage by inducing pro-inflammation. The toll-like receptors (TLRs) may serve a pro-inflammatory role in hyperoxemia during ischemia-reperfusion. Sixteen near-term fetal sheep (132-136 d) were subjected to 10 min of cord occlusion, delivery and mechanical ventilation with 100% O2 (n = 8), or 21% O2 (n = 8) for 30 min followed by normoxemia for 90 min. Eight sheep fetuses were delivered immediately with inspired O2 targeted at normoxemia for 120 min (controls). Levels and distributions of mRNAs for IL-1beta, TNF-alpha, IL-12p40, IL-18, IL-6, IL-10, IFN-gamma, TLR-2, -3 and -4 in cerebral tissue at 2 h after birth were evaluated with real-time polymerase chain reaction (PCR) and in situ hybridization. Expressions of IL-1beta, IL-12p40, TLR-2, and TLR-4 were increased in cortex/subcortex after resuscitation with 100% O2 compared with 21% O2 (all p < 0.05) and to controls (all p < 0.05). Increased cellular expression of IL-1beta was localized to sub-meningeal cortical layers and to sub-cortical white matter. Hyperoxic resuscitation at birth following fetal asphyxia induces a cerebral pro-inflammatory response with an up-regulation of TLR-2 and -4. These may be early events leading to increased tissue damage after exposure to hyperoxemia at birth. |
| | |
Authors:
|
Tina Markus; Stefan Hansson; Isis Amer-Wåhlin; Lena Hellström-Westas; Ola Didrik Saugstad; David Ley |
Related Documents
:
|
6868176 - Hyperbaric exposure during pregnancy in sheep: staged and rapid decompression. 1220656 - Wimmera rye grass toxicity in western australia. 10121256 - Taking your medicare claims to court. 8722286 - Winter poisoning of coyotes and raptors with furadan-laced carcass baits. 6868176 - Hyperbaric exposure during pregnancy in sheep: staged and rapid decompression. 12044856 - Meckel gruber syndrome: a first trimester diagnosis of a recurrent case. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Pediatric research Volume: 62 ISSN: 0031-3998 ISO Abbreviation: Pediatr. Res. Publication Date: 2007 Jul |
Date Detail:
|
Created Date: 2007-06-21 Completed Date: 2007-09-26 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0100714 Medline TA: Pediatr Res Country: United States |
Other Details:
|
Languages: eng Pagination: 71-7 Citation Subset: IM |
Affiliation:
|
Department of Pediatrics, Lund University, 221 85 Lund, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn Asphyxia* Cerebral Cortex / cytology, immunology*, pathology Cytokines / genetics, immunology Female Fetus / physiology* Inflammation / metabolism* Interleukin-12 Subunit p40 / genetics, metabolism Interleukin-1beta / genetics, metabolism Molecular Sequence Data Pregnancy Resuscitation* Sheep, Domestic Toll-Like Receptors / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; 0/Interleukin-12 Subunit p40; 0/Interleukin-1beta; 0/Toll-Like Receptors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Knowledge of reproductive physiology and hormone therapy in 53- to 54-year-old Swedish women: a popu...
Next Document: Occupational exposure to low concentrations of carbon monoxide and sleep disordered breathing. A neg...