Document Detail

Cerebral blood flow autoregulation during hypobaric hypotension assessed by laser Doppler scanning.
MedLine Citation:
PMID:  7929652     Owner:  NLM     Status:  MEDLINE    
Hypobaric hypotension was used to reduce systemic blood pressure in rats below the lower threshold of CBF autoregulation to evaluate a new laser Doppler (LD) "scanning" technique. Spontaneously breathing male Wistar Kyoto rats (n = 8) were anesthetized with chloral hydrate and the head fixed in a stereotaxic head holder. A cranial window with intact dura mater was introduced to assess local CBF (lCBF) by LD. One stationary probe served to detect rapid flow changes, whereas the second probe was used to sample lCBF recordings from many cortical locations by means of a stepping motor-controlled micromanipulator to obtain lCBF frequency histograms. Advantages are an improved spatial resolution together with the easy detection of low-flow areas and a better comparison of data from individual experiments. Arterial blood pressure was stepwise reduced by exposing the lower body portions to subatmospheric pressures (hypobaric hypotension), thus avoiding the use of drugs or heparinization. The lower threshold of CBF autoregulation was detected by "scanning" at arterial pressures between 50 and 46 mm Hg, with low-flow spots occurring immediately. The data suggest LD scanning as a method suited particularly for studies where lCBF inhomogeneities are expected, e.g., the ischemic penumbra or sinus vein thrombosis.
A Heimann; S Kroppenstedt; P Ulrich; O S Kempski
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  14     ISSN:  0271-678X     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1994-11-21     Completed Date:  1994-11-21     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1100-5     Citation Subset:  IM    
Institute for Neurosurgical Pathophysiology, Johnannes Gutenberg University Mainz, Germany.
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MeSH Terms
Atmospheric Pressure*
Blood Pressure
Cerebrovascular Circulation*
Hypotension / physiopathology*
Laser-Doppler Flowmetry / methods*
Rats, Inbred WKY

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