| Cerebral angiogenesis induced by growth factors: intravital microscopic studies using models. | |
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MedLine Citation:
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PMID: 14724336 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pharmacological induction of angiogenesis is a new treatment of cerebrovascular insufficiency without surgical treatment. It is an urgent task to investigate the dynamic process of angiogenesis and of the microvascular perfusion of the cerebral neoplastic tissue in vivo. The present study is concerned with microcirculatory aspects of cerebral neocapillaries in vivo. A novel model of cerebral angiogenesis was developed by inducing cerebral neocapillaries in mice using growth factors such as basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF). By a direct observation of the neocapillary microcirculation under a fluorescence videomicroscope, the neocapillary density, diameter and red cell velocity were measured to evaluate the development and remodeling of the neocapillaries with the number of days after incubation. The neocapillary response to topically applied acetylcholine (ACh) was examined by measuring changes in the diameter and red cell velocity. It was shown that PDGF-induced neocapillaries was dilated in response to ACh on day 28 after incubation, while bFGF-induced neocapillaries was not. Furthermore, the neocapillary pericytes were observed using confocal laser microscopy, based on the fluorescence immunohistochemical images of the neoplastic tissue. Several pericytes, stained with anti-NG_2, appeared in the neocapillaries. It was suggested that these pericytes might be recruited in the neocapillaries to regulate blood flow without vascular smooth muscle. |
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Authors:
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Hideyuki Niimi |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Clinical hemorheology and microcirculation Volume: 29 ISSN: 1386-0291 ISO Abbreviation: Clin. Hemorheol. Microcirc. Publication Date: 2003 |
Date Detail:
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Created Date: 2004-01-15 Completed Date: 2004-05-19 Revised Date: 2005-11-16 |
Medline Journal Info:
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Nlm Unique ID: 9709206 Medline TA: Clin Hemorheol Microcirc Country: Netherlands |
Other Details:
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Languages: eng Pagination: 149-56 Citation Subset: IM |
Affiliation:
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Department of Vascular Physiology, National Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan. niimi@ri.ncvc.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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pharmacology Angiogenesis Inducing Agents / pharmacology* Animals Blood Flow Velocity Brain / blood supply* Capillaries / drug effects Drug Implants Fibroblast Growth Factor 2 / pharmacology* Mice Microscopy, Confocal Microscopy, Fluorescence Microscopy, Video Models, Animal Neovascularization, Physiologic / drug effects* Pericytes / physiology Platelet-Derived Growth Factor / pharmacology* Receptors, Muscarinic / drug effects, physiology Vasodilation / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Angiogenesis Inducing Agents; 0/Drug Implants; 0/Platelet-Derived Growth Factor; 0/Receptors, Muscarinic; 103107-01-3/Fibroblast Growth Factor 2; 51-84-3/Acetylcholine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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