| Cerebral Metabolism after Forced or Voluntary Physical Exercise. | |
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MedLine Citation:
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PMID: 21396919 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The pathophysiology of stroke, a leading cause of morbidity and mortality, is still in the process of being understood. Pre-ischemic exercise has been known to be beneficial in reducing the severity of stroke-induced brain injury in animal models. Forced exercise with a stressful component, rather than voluntary exercise, was better able to induce neuroprotection. This study further determined the changes in cerebral metabolism resulting from the two methods of exercise (forced versus voluntary). Adult male Sprague Dawley rats were randomly assigned to 3 groups: the control group (no exercise), the forced treadmill exercise group, and the voluntary running wheel exercise group. In order to measure the extent of cerebral metabolism in animals with different exercise regimens, mRNA levels and protein expression of Glucose Transporter 1 and Glucose Transporter 3 (GLUT1 and GLUT3), Phosphofructo-kinase (PFK), Lactate Dehydrogenase (LDH), and Adenosine monophosphate kinase (AMPK) were measured utilizing real-time reverse transcription polymerase chain reaction (PCR) analysis as well as Western blot analysis. Phosphorylated AMPK activity was also measured using an ELISA activity kit, and Hypoxic Inducible Factor (HIF)-1α was measured at transcription and translation levels. The data shows that the forced exercise group had a significant (p<0.05) increase in cerebral glycolysis, including expressions of GLUT1, GLUT3, PFK, LDH, phosphorylated AMPK activity and HIF-1α, when compared to the voluntary exercise and the control groups. Our results suggest that the effects of different exercise on HIF-1α expression and cerebral glycolysis may provide a possible reason for the discrepancy in neuroprotection, with forced exercise faring better than voluntary exercise through increased cerebral metabolism. |
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Authors:
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Harish Kinni; Miao Guo; Jamie Y Ding; Sanjay Konakondla; David Dornbos; Raymond Tran; Murali Guthikonda; Yuchuan Ding |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-9 |
Journal Detail:
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Title: Brain research Volume: - ISSN: 1872-6240 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2010. Published by Elsevier B.V. |
Affiliation:
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Department of Neurosurgery, Wayne State University School of Medicine, Detroit, MI. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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