| Cerebellar Purkinje Cell Neurodegeneration After Cardiac Arrest: Effect of Therapeutic Hypothermia. | |
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MedLine Citation:
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PMID: 22683500 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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AIMS: : The cerebellum is among the brain regions most vulnerable to damage caused by cardiac arrest, and cerebellar Purkinje cell loss may contribute to neurologic dysfunction, including post-hypoxic myoclonus. However, it remains unknown whether cerebellar Purkinje cells are protected by post-cardiac arrest therapeutic hypothermia (TH). Therefore, we examined the effect of post-cardiac arrest TH onset and duration on cerebellar Purkinje cell loss. METHODS: : Samples from a previously published study of post-cardiac arrest TH were utilized for the present analysis. Adult male rats subjected to asphyxial cardiac arrest and cardiopulmonary resuscitation were block randomized to normothermia (37.0°C) or TH (33.0°C) initiated 0, 1, 4, or 8hours after return of spontaneous circulation (ROSC) and maintained for 24 or 48hours. Cerebella from rats surviving 7 days after ROSC were processed for histology and immunohistochemistry. Purkinje cell density was quantified in Nissl-stained sections of the primary fissure of the cerebellar vermis. RESULTS: : With post-cardiac arrest normothermia, Purkinje cell density in the primary fissure was severely reduced compared to sham-injured controls (3.8±1.8 cells/mm vs. 35.9±2.4 cells/mm, p<0.001). TH moderately improved Purkinje cell survival in all groups combined (14.0±5.6 cells/mm, p<0.001 compared to normothermia). There was no statistical difference in Purkinje cell protection based on TH onset time or duration. CONCLUSION: : These results indicate that post-cardiac arrest TH protects selectively vulnerable cerebellar Purkinje cells within a broad therapeutic window. The potential clinical implications for improving Purkinje cell survival require further investigation. |
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Authors:
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Michael Gregory Paine; Dongfang Che; Luchuan Li; Robert William Neumar |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-6 |
Journal Detail:
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Title: Resuscitation Volume: - ISSN: 1873-1570 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-6-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0332173 Medline TA: Resuscitation Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Ireland Ltd. |
Affiliation:
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Center for Resuscitation Science, Department of Emergency Medicine, Perelman School of Medicine, University of Pennsylvania. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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