| Ceramides stimulate caspase-14 expression in human keratinocytes. | |
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MedLine Citation:
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PMID: 23362869 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Caspase-14 is an enzyme that is expressed predominantly in cornifying epithelia and catalyses the degradation of profilaggrin. Additionally, caspase-14 plays an important role in the terminal differentiation of keratinocytes. However, how caspase-14 expression is regulated remains largely unknown. Here we demonstrate that ceramides (C(2) -Cer and C(6) -Cer), but not other sphingolipids (C(8) -glucosylceramides, sphinganine, sphingosine-1-phosphate or ceramide-1-phosphate), increase caspase-14 expression (mRNA and protein) in cultured human keratinocytes in a dose- and time-dependent manner. Inhibitors of glucosylceramide synthase and ceramidase increase endogenous ceramide levels and also increase caspase-14 expression, indicating an important regulatory role for ceramides and suggesting that the conversion of ceramides to other metabolites is not required. The increase in caspase-14 expression induced by ceramides is first seen at 16 h and requires new protein synthesis, suggesting that the ceramide-induced increase is likely an indirect effect. Furthermore, ceramides increase caspase-14 gene expression primarily by increasing transcription. Blocking de novo synthesis of ceramides does not affect caspase-14 expression, suggesting that basal expression is not dependent on ceramide levels. These studies show that ceramides, an important structural lipid, stimulate caspase-14 expression providing a mechanism for coordinately regulating the formation of lipid lamellar membranes with the formation of corneocytes. |
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Authors:
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Yan J Jiang; Peggy Kim; Yoshikazu Uchida; Peter M Elias; Daniel D Bikle; Carl Grunfeld; Kenneth R Feingold |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Experimental dermatology Volume: 22 ISSN: 1600-0625 ISO Abbreviation: Exp. Dermatol. Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-01-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9301549 Medline TA: Exp Dermatol Country: Denmark |
Other Details:
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Languages: eng Pagination: 113-8 Citation Subset: IM |
Copyright Information:
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© 2012 John Wiley & Sons A/S. |
Affiliation:
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Metabolism Section, Veterans Affairs Medical Center, Northern California Institute for Research and Education, University of California at San Francisco, San Francisco, CA, USA; Endocrinology Section, Veterans Affairs Medical Center, Northern California Institute for Research and Education, University of California at San Francisco, San Francisco, CA, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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